Molecular Evolution across Mouse Spermatogenesis.
Molecular Evolution across Mouse Spermatogenesis.
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DOI：
10.1093/molbev/msac023
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发表时间：
2022-02-03
影响因子：
10.7
通讯作者：
Good JM
中科院分区：
文献类型：
--
作者：
Kopania EEK;Larson EL;Callahan C;Keeble S;Good JM
参与精子发生的基因往往进化得很快，但我们缺乏对蛋白质序列和基因表达模式如何在这一复杂的发育过程中进化的清楚了解。我们使用荧光激活细胞分类(FAC)来生成跨越∼7My进化的13个近交系小鼠(MU)的早期(减数分裂)和晚期(减数分裂后)细胞类型的表达数据。我们使用这些比较的发育数据来研究谱系特异性表达、蛋白质编码序列和表达水平的演变。我们发现，在精子发生后期，谱系特异性增强，蛋白质编码和表达差异更快，这表明快速睾丸分子进化的标志在精子发育过程中时有发生。尽管分子进化的这些组成部分在总体上具有很强的发育相似性，但蛋白质和表达差异在不同基因之间的相关性很弱。我们发现与常染色体相比，X染色体上的蛋白质进化更快，而X连锁基因的表达往往相对保守，可能反映了染色体特有的调控限制。利用四个菌株之间杂交的等位基因特异性FACS表达数据，我们发现不同调控机制的相对贡献也因细胞类型而异。显示顺式调控变化的基因在精子发生的后期更为常见，并倾向于与更大的表达水平差异和物种之间的表达差异有关。相比之下，具有反式作用变化的基因在早期更常见，而且往往在不同物种之间更保守。我们的发现促进了对精子发生过程中基因进化的理解，并强调了发育背景在分子进化研究中的基本重要性。
Genes involved in spermatogenesis tend to evolve rapidly, but we lack a clear understanding of how protein sequences and patterns of gene expression evolve across this complex developmental process. We used fluorescence-activated cell sorting (FACS) to generate expression data for early (meiotic) and late (postmeiotic) cell types across 13 inbred strains of mice (Mus) spanning ∼7 My of evolution. We used these comparative developmental data to investigate the evolution of lineage-specific expression, protein-coding sequences, and expression levels. We found increased lineage specificity and more rapid protein-coding and expression divergence during late spermatogenesis, suggesting that signatures of rapid testis molecular evolution are punctuated across sperm development. Despite strong overall developmental parallels in these components of molecular evolution, protein and expression divergences were only weakly correlated across genes. We detected more rapid protein evolution on the X chromosome relative to the autosomes, whereas X-linked gene expression tended to be relatively more conserved likely reflecting chromosome-specific regulatory constraints. Using allele-specific FACS expression data from crosses between four strains, we found that the relative contributions of different regulatory mechanisms also differed between cell types. Genes showing cis-regulatory changes were more common late in spermatogenesis, and tended to be associated with larger differences in expression levels and greater expression divergence between species. In contrast, genes with trans-acting changes were more common early and tended to be more conserved across species. Our findings advance understanding of gene evolution across spermatogenesis and underscore the fundamental importance of developmental context in molecular evolutionary studies.
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影响因子：
2.7
作者：
Borowiec ML
通讯作者：
Borowiec ML
DOI：
10.1073/pnas.1705601114
发表时间：
2017-07-25
影响因子：
11.1
作者：
Alavioon, Ghazal;Hotzy, Cosima;Immler, Simone
通讯作者：
Immler, Simone
影响因子：
7
作者：
Coolon JD;McManus CJ;Stevenson KR;Graveley BR;Wittkopp PJ
通讯作者：
Wittkopp PJ
DOI：
10.1007/s00335-017-9704-9
发表时间：
2017-10
期刊：
Mammalian genome : official journal of the International Mammalian Genome Society
影响因子：
--
作者：
Chang PL;Kopania E;Keeble S;Sarver BAJ;Larson E;Orth A;Belkhir K;Boursot P;Bonhomme F;Good JM;Dean MD
通讯作者：
Dean MD
DOI：
10.1093/database/baaa035
发表时间：
2020-06-04
影响因子：
5.8
作者：
Alanis-Lobato, Gregorio;Mollmann, Jannik S.;Andrade-Navarro, Miguel A.
通讯作者：
Andrade-Navarro, Miguel A.