Digging behavior discrimination test to probe burrowing and exploratory digging in male and female mice.
Digging behavior discrimination test to probe burrowing and exploratory digging in male and female mice.
复制标题
挖掘行为辨别试验，以探索雄性和雌性小鼠的洞穴和探索性挖掘。
DOI：
10.1002/jnr.24857
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发表时间：
2021-09
影响因子：
4.2
通讯作者：
Manzini, M. Chiara
中科院分区：
文献类型：
--
作者：
Pond, Heather L.;Heller, Abigail T.;Gural, Brian M.;McKissick, Olivia P.;Wilkinson, Molly K.;Manzini, M. Chiara
关键词：
挖掘行为常用于测试小鼠的运动功能和重复行为。不同的挖掘范式已经开发了与焦虑和强迫相关的行为，在小鼠品系中产生重演导致精神和神经疾病的基因突变。然而，这些测试的解释一直被确定小鼠挖掘背后的动机的困难所混淆。挖掘是一种自然主义的老鼠行为，可以专注于不同的目标，即觅食，挖掘庇护所，埋葬物体，甚至是娱乐，如狗，雪貂和人类儿童。然而，从目前的测试协议的结果的解释假设的动机背后的行为往往得出结论，增加挖掘是一种重复或强迫行为。我们问是否提供不同类型的挖掘活动之间的选择将增加敏感性，以评估挖掘动机。在这里，我们提出了一个测试来区分小鼠的穴居和探索性挖掘。我们发现，当有选择时，老鼠更喜欢打洞。当食物限制被用来促进从洞穴到探索的转换时，雄性很容易从洞穴转换到外面挖掘，而雌性则没有。此外，当我们测试一个智力残疾和自闭症谱系障碍的模型时，在大理石掩埋测试中显示出不一致的结果，即Cc2d1a条件性敲除小鼠，我们发现只有雄性小鼠的穴居能力大大降低。我们的研究结果表明，挖掘是一种微妙的动机行为，并表明雄性和雌性啮齿动物可能会有不同的表现。
Digging behavior is often used to test motor function and repetitive behaviors in mice. Different digging paradigms have been developed for behaviors related to anxiety and compulsion in mouse lines generated to recapitulate genetic mutations leading to psychiatric and neurological disorders. However, the interpretation of these tests has been confounded by the difficulty of determining the motivation behind digging in mice. Digging is a naturalistic mouse behavior, that can be focused toward different goals, i.e. foraging for food, burrowing for shelter, burying objects, or even for recreation as has been shown for dogs, ferrets, and human children. However, the interpretation of results from current testing protocols assumes the motivation behind the behavior often concluding that increased digging is a repetitive or compulsive behavior. We asked whether providing a choice between different types of digging activities would increase sensitivity to assess digging motivation. Here, we present a test to distinguish between burrowing and exploratory digging in mice. We found that mice prefer burrowing when the option is available. When food restriction was used to promote a switch from burrowing to exploration, males readily switched from burrowing to digging outside, while females did not. In addition, when we tested a model of intellectual disability and autism spectrum disorder that had shown inconsistent results in the marble burying test, the Cc2d1a conditional knock-out mouse, we found greatly reduced burrowing only in males. Our findings indicate that digging is a nuanced motivated behavior and suggest that male and female rodents may perform it differently.
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影响因子：
3
作者：
Jirkof, Paulin
通讯作者：
Jirkof, Paulin
影响因子：
5
作者：
BROEKKAMP, CL;RIJK, HW;LLOYD, KL
通讯作者：
LLOYD, KL
影响因子：
2.7
作者：
Dell'Omo, G;Ricceri, L;Lipp, HP
通讯作者：
Lipp, HP
DOI：
10.1152/ajpregu.2000.279.6.r2048
发表时间：
2000-12-01
影响因子：
2.8
作者：
Díaz-Muñoz, M;Vázquez-Martínez, O;Escobar, C
通讯作者：
Escobar, C
影响因子：
3.8
作者：
Bakdash JZ;Marusich LR
通讯作者：
Marusich LR