Gene expression relationship between prostate cancer cells of Gleason 3, 4 and normal epithelial cells as revealed by cell type-specific transcriptomes.
Gene expression relationship between prostate cancer cells of Gleason 3, 4 and normal epithelial cells as revealed by cell type-specific transcriptomes.
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DOI：
10.1186/1471-2407-9-452
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发表时间：
2009-12-18
期刊：
影响因子：
3.8
通讯作者：
Liu AY
中科院分区：
文献类型：
--
作者：
Pascal LE;Vêncio RZ;Page LS;Liebeskind ES;Shadle CP;Troisch P;Marzolf B;True LD;Hood LE;Liu AY
原发性肿瘤中的前列腺癌细胞已被分类为CD10-/CD13-/CD24hi/CD26+/CD38lo/CD44-/CD104-。这种 CD 表型表明癌细胞和管腔细胞之间存在谱系关系。肿瘤的格里森分级是对肿瘤腺体分化的描述。较高的格里森评分与治疗失败相关。通过细胞分选从 Gleason 3+3 (G3) 和 Gleason 4+4 (G4) 肿瘤中分离 CD26+ 癌细胞，并通过 Affymetrix DNA 阵列分析确定其基因表达或转录组。使用数据集分析来确定G3和G4之间以及前列腺癌细胞系和组织学上正常的前列腺管腔细胞之间的基因表达相似性和差异。将 G3 和 G4 转录组与非癌症前列腺细胞类型（包括腔细胞、基底细胞、基质纤维肌肉细胞和内皮细胞）的转录组进行比较。各种转录组数据集的主成分分析表明，luminal 和 G3 之间的关系比 luminal 和 G4 之间的关系更密切。数据集比较还表明，癌症转录组与前列腺癌细胞系的转录组存在显着差异。癌症中差异表达的基因是癌症检测的潜在生物标志物，而 G3 和 G4 之间差异表达的基因是疾病分层的潜在生物标志物，因为 G4 癌症与不良预后相关。差异表达的基因可能有助于前列腺癌表型并构成这些特定癌细胞类型的特征。
Prostate cancer cells in primary tumors have been typed CD10-/CD13-/CD24hi/CD26+/CD38lo/CD44-/CD104-. This CD phenotype suggests a lineage relationship between cancer cells and luminal cells. The Gleason grade of tumors is a descriptive of tumor glandular differentiation. Higher Gleason scores are associated with treatment failure. CD26+ cancer cells were isolated from Gleason 3+3 (G3) and Gleason 4+4 (G4) tumors by cell sorting, and their gene expression or transcriptome was determined by Affymetrix DNA array analysis. Dataset analysis was used to determine gene expression similarities and differences between G3 and G4 as well as to prostate cancer cell lines and histologically normal prostate luminal cells. The G3 and G4 transcriptomes were compared to those of prostatic cell types of non-cancer, which included luminal, basal, stromal fibromuscular, and endothelial. A principal components analysis of the various transcriptome datasets indicated a closer relationship between luminal and G3 than luminal and G4. Dataset comparison also showed that the cancer transcriptomes differed substantially from those of prostate cancer cell lines. Genes differentially expressed in cancer are potential biomarkers for cancer detection, and those differentially expressed between G3 and G4 are potential biomarkers for disease stratification given that G4 cancer is associated with poor outcomes. Differentially expressed genes likely contribute to the prostate cancer phenotype and constitute the signatures of these particular cancer cell types.
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影响因子：
3.2
作者：
Hudson, DL;Guy, AT;Masters, JRW
通讯作者：
Masters, JRW
影响因子：
6.6
作者：
Liu, AY;Zhang, H;Diamond, DL
通讯作者：
Diamond, DL
DOI：
10.1073/pnas.94.20.10705
发表时间：
1997-09-30
影响因子：
11.1
作者：
Liu, AY;True, LD;vandenEngh, G
通讯作者：
vandenEngh, G
影响因子：
6
作者：
Liu, AY;True, LD
通讯作者：
True, LD
影响因子：
4.7
作者：
Inokuchi, Junichi;Lau, Alice;Ornstein, David K.
通讯作者：
Ornstein, David K.