Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates.
Epidemiological and genomic characteristics of global mcr-positive Escherichia coli isolates.
复制标题
全球 mcr 阳性大肠杆菌分离株的流行病学和基因组特征
DOI：
10.3389/fmicb.2022.1105401
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发表时间：
2022
影响因子：
5.2
通讯作者：
Shen, Han
中科院分区：
文献类型：
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作者：
Shi, Jiping;Zhu, Hong;Liu, Chang;Xie, Hui;Li, Chuchu;Cao, Xiaoli;Shen, Han
目的探讨大肠埃希菌耐多粘菌素株在世界范围内的分布。大肠杆菌）危害公众健康。本研究的目的是更好地了解全球E.大肠杆菌分离株携带动员粘菌素抗性（mcr）基因，提供信息，以协助感染和预防。方法从NCBI下载大肠埃希菌基因组，采用BLASTP法检测mcr。Per软件用于从GenBank中提取宿主、资源、收集数据和来源国等信息。分析了这些基因组中的序列类型（ST）、质粒流行率、抗菌素耐药基因（ARG）和毒力因子（VF）。进行统计分析以评估mcr、ARG、质粒和ST之间的关系。结果共鉴定出778株mcr阳性菌株。检测到4种mcr变体，mcr-1（86.1%）最广泛，其次是mcr-9（5.7%）、mcr-5（4.4%）和mcr-3（3.0%）。确定了多种ARG，其中blaCTX-M（53.3%）、fosA（28.8%）、qnr（26.1%）、blaNDM（19.8%）和aac（6 '）-Ib-cr（14.5%）最常见。总的来说，确定了239种不同的ST，其中ST 10（13.8%）是最普遍的。共检出113种VF，其中terC（99.9%）和gad（83.0%）最常见。共鉴定出20种质粒; IncFIB（64.1%）、IncX（42.3%）和IncX（42.3%）是最常见的复制子。IncI 2和IncX 4常在mcr-1阳性分离株中检出，而IncFII、IncI 1-I和IncHI 2分别是mcr-3、mcr-5和mcr-9阳性分离株中的优势质粒。在ST 156和ST 131分离株中观察到较高的ARG和VF频率。结论半数以上的mcr阳性大肠埃希氏菌感染了大肠杆菌。大肠杆菌菌株携带地方性ARG和VF。鉴于ST 10的快速传播以及ST 156中ARG和VF的融合，ST 10和ST 156分离株值得进一步关注。
Objective The worldwide dissemination of colistin-resistant Escherichia coli (E. coli) endangers public health. This study aimed to better understand the global genomic epidemiology of E. coli isolates carrying mobilized colistin resistance (mcr) genes, providing information to assist in infection and prevention. Methods Escherichia coli genomes were downloaded from NCBI, and mcr was detected using BLASTP. Per software was used to extract information on hosts, resources, collection data, and countries of origin from GenBank. Sequence types (STs), prevalence of plasmids, antimicrobial resistance genes (ARG), and virulence factors (VF) in these genomes were analyzed. Statistical analyses were performed to assess the relationships between mcr, ARGs, plasmids, and STs. Results In total, 778 mcr-positive isolates were identified. Four mcr variants were detected, with mcr-1 (86.1%) being the most widespread, followed by mcr-9 (5.7%), mcr-5 (4.4%), and mcr-3 (3.0%). Multiple ARGs were identified, with blaCTX–M (53.3%), fosA (28.8%), qnr (26.1%), blaNDM (19.8%), and aac (6’)-Ib-cr (14.5%) being the most common. Overall, 239 distinct STs were identified, of which ST10 (13.8%) was the most prevalent. A total of 113 different VFs were found, terC (99.9%) and gad (83.0%) were most frequently detected. Twenty types of plasmids were identified; IncFIB (64.1%), IncX (42.3%), and IncX (42.3%) were the most common replicons. IncI2 and IncX4 were frequently detected in mcr-1-positive isolates, whereas IncFII, IncI1-I, and IncHI2 were dominant plasmids in mcr-3, mcr-5, and mcr-9-positive isolates, respectively. A higher frequency of ARGs and VFs was observed among ST156 and ST131 isolates. Conclusion Our data indicated that more than half of the mcr-positive E. coli strains carried endemic ARGs and VFs. ST10 and ST156 isolates deserved further attention, given the rapid transmission of ST10 and the convergence of ARGs and VFs in ST156.
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影响因子：
14.9
作者：
Kim T;Seo HD;Hennighausen L;Lee D;Kang K
通讯作者：
Kang K
影响因子：
3.2
作者：
Khine NO;Lugsomya K;Kaewgun B;Honhanrob L;Pairojrit P;Jermprasert S;Prapasarakul N
通讯作者：
Prapasarakul N
影响因子：
2.2
作者：
Alangari, Abdulaziz;Jaffal, Ahmad Abu;Alyousef, Abdulaziz M. M.;Alyousef, Abdullah A. A.
通讯作者：
Alyousef, Abdullah A. A.
影响因子：
3.7
作者：
通讯作者：
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全球流行病学，遗传环境，危险因素和MCR基因的治疗前景：当前和新兴的更新。
影响因子：
5.7
作者：
通讯作者：
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