Chicken Protein S Gene Regulates Adipogenesis and Affects Abdominal Fat Deposition.
Chicken Protein S Gene Regulates Adipogenesis and Affects Abdominal Fat Deposition.
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DOI：
10.3390/ani12162046
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发表时间：
2022-08-11
期刊：
影响因子：
3
通讯作者：
Nie, Qinghua
中科院分区：
文献类型：
--
作者：
Guo, Lijin;Huang, Weiling;Zhang, Siyu;Huang, Yulin;Xu, Yibin;Wu, Ruiquan;Fang, Xiang;Xu, Haiping;Nie, Qinghua
随着健康饮食的日益普及，低脂肉类越来越受到大众的青睐，而腹部脂肪的过度积累又增加了肉鸡养殖业的成本，这些都促使肉鸡业的养殖变革。从遗传角度研究细胞水平的脂肪积累将有助于我们了解基因介导的鸡腹部脂肪积累。本研究旨在探讨PROS1基因在脂肪细胞中的作用及其在肉鸡育种中的应用前景。根据我们的研究结果，我们发现PROS1基因可以促进脂肪细胞增殖，并可以在细胞水平上减少脂肪沉积，并且其突变与鸡的脂肪性状高度相关。 （1）背景：肉鸡腹部脂肪沉积过多，不仅造成饲料浪费，还会引发一系列代谢性疾病。提高生长率、降低腹脂率已逐渐成为肉鸡行业新的养殖目标。在之前的一项研究中，PROS1 在低腹脂肪肉鸡中高表达，表明其在肉鸡脂肪形成中具有潜在作用。然而，PROS1 在前脂肪细胞中的功能及其与腹部脂肪特征的关联仍需确定。 (2)方法：采用qRT-PCR和Western Blot在RNA和蛋白水平上定量基因表达；流式细胞术和EdU检测细胞增殖情况； GLM 分析用于确定 PROS1 SNP 与胴体性状之间的关联。 (3)结果：PROS1在高腹脂肪鸡中表达下调； PROS1促进前脂肪细胞增殖，但抑制前脂肪细胞分化； PROS1 5'侧翼的SNP与腹部脂肪重量率显着相关。 (4)结论：鸡PROS1具有抑制脂肪生成的作用，其多态性与腹部脂肪重量率相关，可作为鸡育种的分子标记，表明PROS1是调控腹部脂肪沉积的有效潜在基因。
Low-fat meat is increasingly desired by the public due to the growing popularity of healthy diets, and the excessive accumulation of abdominal fat increases costs in the broiler breeding industry, all of which have encouraged breeding changes in the broiler industry. Investigating fat accumulation at a cellular level from a genetic perspective will help us understand gene-mediated abdominal fat accumulation in chickens. This study aimed to explore the role of the PROS1 gene in adipose cells and its application prospect in broiler breeding. Based on our findings, we found that the PROS1 gene can contribute to adipose cell proliferation and can reduce fat deposits at the cellular level, and its mutations are highly correlated with chicken fat traits. (1) Background: Excessive abdominal fat deposition in broilers not only causes feed waste but also leads to a series of metabolic diseases. It has gradually become a new breeding goal of the broiler industry to improve growth rates and to reduce abdominal fat rates. In a previous study, PROS1 was highly expressed in low-abdominal fat broilers, suggesting a potential role in broilers adipogenesis. However, the function of PROS1 in preadipocytes and its association with abdominal fat traits need to be characterized. (2) Methods: qRT-PCR and Western Blot were used to quantify gene expression at the RNA and protein levels; flow cytometry and EdU were carried out to detect cell proliferation; and a GLM analysis was used to determine the association between PROS1 SNPs and carcass traits. (3) Results: PROS1 was downregulated in high-abdominal fat chicken; PROS1 contributed preadipocyte proliferation but suppressed preadipocyte differentiation; and the SNPs in the PROS1 5′ flank were significantly associated with the abdominal fat weight rate. (4) Conclusions: Chicken PROS1 is able to suppress adipogenesis, and its polymorphisms are associated with the abdominal fat weight rate, which can be considered the molecular markers for chicken breeding, indicating that PROS1 is an effective potential gene in regulating abdominal fat deposition.
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