Characterization of the Dioscorin Gene Family in Dioscorea alata Reveals a Role in Tuber Development and Environmental Response.
Characterization of the Dioscorin Gene Family in Dioscorea alata Reveals a Role in Tuber Development and Environmental Response.
复制标题
薯蓣苷基因家族的表征揭示了其在块茎发育和环境响应中的作用
DOI：
10.3390/ijms18071579
复制
发表时间：
2017-07-20
影响因子：
5.6
通讯作者：
Huang D
中科院分区：
文献类型：
--
作者：
Liu L;Huang Y;Huang X;Yang J;Wu W;Xu Y;Cong Z;Xie J;Xia W;Huang D
薯蓣皂苷是薯蓣块茎中主要的可溶性蛋白质之一。与其他众所周知的植物贮藏蛋白如马铃薯糖蛋白和孢子素不同，薯蓣皂苷被认为是贮藏蛋白，其表达复杂性的分子机制尚不清楚。本研究中，我们从拟南芥阿拉塔L.中分离了5个薯蓣皂苷基因，包括三种A类（Da-dio 1、-3和-4）和两种B类（Da-dio 2和-5）亚型。随着山药的萌发和再生，所有薯蓣皂苷基因的表达量在母薯中逐渐降低。另一方面，所有薯蓣皂苷基因积累的成绩单逐步与新块茎块茎的块茎发展，Da-dio 5是最突出的异构体。在山药叶片中，5种激素（赤霉素、水杨酸、吲哚乙酸、脱落酸和乙烯）和3种非生物胁迫（高温、低温和干旱）均上调Da-dio 5基因的表达。为了进一步阐明Da-dio 5表达的调控机制，产生了含有Da-dio 5启动子-β-葡萄糖醛酸酶（GUS）融合体的转基因拟南芥植物。GUS染色结果表明，Da-dio 5启动子主要在茎尖分生组织（SAM）和下胚轴中表达，并受到5种激素和上述3种非生物胁迫的诱导。这些结果表明Da-dio 5在山药发芽、再生和块茎形成中以及对环境线索的响应中具有不同的作用。
Dioscorin is one of the major soluble proteins in yam tubers. Unlike other well-known plant storage proteins, such as patatin and sporamin, dioscorin is argued for its function as storage proteins, and the molecular mechanisms underlying its expressional complexity are little understood. In this study, we isolated five dioscorin genes from Dioscorea alata L., comprising three class A (Da-dio1, -3 and -4) and two class B (Da-dio2 and -5) isoforms. Expressions of all dioscorin genes gradually decreased in mother tubers during yam sprouting and regrowth. On the other hand, all dioscorin genes accumulated transcripts progressively with tuber development in new tubers, with Da-dio5 being the most prominent isoform. In yam leaves, the expressions of Da-dio5 were up-regulated by the treatments of five phytohormones (gibberellic acid, salicylic acid, indole-3-acetic acid, abscisic acid, and ethylene), and three abiotic stresses (high-temperature, low-temperature and drought). To further elucidate the regulatory mechanisms of Da-dio5 expressions, transgenic Arabidopsis plants harboring the Da-dio5 promoter-β-glucuronidase (GUS) fusion were generated. GUS staining showed that expressions of the Da-dio5 promoter were detected mainly in the shoot apical meristem (SAM) and hypocotyls, and enhanced by the treatments of the five hormones, and the three abiotic stresses mentioned above. These results suggest diverse roles of Da-dio5 in yam sprouting, regrowth, and tuberization, as well as in response to enviromental cues.
登录
查看更多内容
影响因子：
11.4
作者：
ROSAHL, S;SCHELL, J;WILLMITZER, L
通讯作者：
WILLMITZER, L
影响因子：
6.1
作者：
Hou, WC;Liu, JS;Lin, YH
通讯作者：
Lin, YH
影响因子：
6.4
作者：
HANNAPEL, DJ
通讯作者：
HANNAPEL, DJ
影响因子：
7.4
作者：
PAIVA, E;LISTER, RM;PARK, WD
通讯作者：
PARK, WD
影响因子：
6.1
作者：
Araghiniknam, M;Chung, S;Watson, R R
通讯作者：
Watson, R R