Characterization of luteinizing hormone and luteinizing hormone receptor and their indispensable role in the ovulatory process of the medaka.
Characterization of luteinizing hormone and luteinizing hormone receptor and their indispensable role in the ovulatory process of the medaka.
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DOI：
10.1371/journal.pone.0054482
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Takahashi T
中科院分区：
文献类型：
--
作者：
Ogiwara K;Fujimori C;Rajapakse S;Takahashi T
青鳉（Oryzias latipes）是研究排卵的理想动物模型，但其促黄体生成激素（Lh）及其受体（Lhcgrbb）的分子特性和作用尚未见报道。在这里，我们的青鳉LH/Lhcgrbb系统的特点，注意它的参与排卵过程中的硬骨鱼。在青鳉卵巢中，卵泡刺激素受体mRNA在小型和中型卵泡中表达，而lhcgrbb mRNA在所有生长卵泡的卵泡层中表达。使用HEK 293 T细胞在体外表达青鳉Lhcgrbb的实验显示，来自怀孕母马血清的促性腺激素和青鳉重组Lh（rLh）结合到鱼Lhcgrbb。鱼促性腺激素亚基Gtha，FSHB，和LHB基本上是在相当恒定的水平在24小时产卵周期的鱼的垂体中表达。利用青鳉rLh，我们开发了一个卵泡培养系统，使我们能够跟踪卵母细胞体外成熟和排卵的整个过程。这种卵泡培养方法使我们能够确定排卵前卵泡的LH峰发生在体内排卵前19至15小时之间。本研究还表明，卵母细胞的成熟和排卵在体外比在体内延迟几个小时。在体外用青鳉rLh处理大卵泡显著增加了Mmp 15的表达，Mmp 15以前被证明对鱼的排卵至关重要。这些发现表明，Lh/Lhcgrbb是关键参与诱导卵母细胞成熟和排卵。
The molecular properties and roles of luteinizing hormone (Lh) and its receptor (Lhcgrbb) have not been studied for the medaka (Oryzias latipes), which is an excellent animal model for ovulation studies. Here, we characterized the medaka Lh/Lhcgrbb system, with attention to its involvement in the ovulatory process of this teleost fish. In the medaka ovary, follicle-stimulating hormone receptor mRNA was expressed in small and medium-sized follicles, while lhcgrbb mRNA was expressed in the follicle layers of all growing follicles. Experiments using HEK 293T cells expressing medaka Lhcgrbb in vitro revealed that gonadotropin from pregnant mare’s serum and medaka recombinant Lh (rLh) bound to the fish Lhcgrbb. The fish gonadotropin subunits Gtha, Fshb, and Lhb were essentially expressed at fairly constant levels in the pituitary of the fish during a 24-h spawning cycle. Using medaka rLh, we developed a follicle culture system that allowed us to follow the whole process of oocyte maturation and ovulation in vitro. This follicle culture method enabled us to determine that the Lh surge for the preovulatory follicle occurred in vivo between 19 and 15 h before ovulation. The present study also showed that oocyte maturation and ovulation were delayed several hours in vitro compared with in vivo. Treatment of large follicles with medaka rLh in vitro significantly increased the expression of Mmp15, which was previously demonstrated to be crucial for ovulation in the fish. These findings demonstrate that Lh/Lhcgrbb is critically involved in the induction of oocyte maturation and ovulation.
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DOI：
10.1186/1477-7827-8-34
发表时间：
2010-04-12
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Crespo D;Bonnet E;Roher N;MacKenzie SA;Krasnov A;Goetz FW;Bobe J;Planas JV
通讯作者：
Planas JV
影响因子：
4.1
作者：
Fujimori, Chika;Ogiwara, Katsueki;Takahashi, Takayuki
通讯作者：
Takahashi, Takayuki
影响因子：
2.9
作者：
Goetz, FW;Garczynski, M
通讯作者：
Garczynski, M
影响因子：
4
作者：
Hazzard, TM;Molskness, TA;Stouffer, RL
通讯作者：
Stouffer, RL
影响因子：
3.6
作者：
Bogerd, J;Blomenröhr, M;Schulz, RW
通讯作者：
Schulz, RW