Characterisation of spin coated engineered Escherichia coli biofilms using atomic force microscopy.
Characterisation of spin coated engineered Escherichia coli biofilms using atomic force microscopy.
复制标题
使用原子力显微镜表征旋涂工程大肠杆菌生物膜。
DOI：
10.1016/j.colsurfb.2011.09.007
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Tsoligkas AN
中科院分区：
文献类型：
--
作者：
Tsoligkas AN
生物膜耐受化学和物理极端情况的能力使其有可能被开发为强大的生物催化剂。这一问题的关键是它们承受生物反应器内物理环境的能力；为了评估这种能力，需要了解它们的表面属性和附着强度。利用原子力显微镜在生长条件(30°C)下对大肠杆菌生物膜进行了表征，这种生物膜是通过最近开发的旋涂方法在聚L赖氨酸涂层玻璃衬底上生成的。在整个生物膜发育过程中获得了高分辨率的地形图像，量化了10天成熟过程中尖端-细胞的相互作用力。在3天内，硅原子力显微镜针尖与生物膜表面的粘附力从0.8nN增加到40nN。这很可能是由于在成熟期内产生了胞外聚合物(EPS)，这也是通过电子显微镜观察到的。在成熟后期，也发现了与生物膜表面特征相对应的多个回缩事件，被认为是EPS组分。结果表明，旋涂生物膜的表面附着力强于相同玻璃基板上同等的常规生物膜。
The ability of biofilms to withstand chemical and physical extremes gives them the potential to be developed as robust biocatalysts. Critical to this issue is their capacity to withstand the physical environment within a bioreactor; in order to assess this capability knowledge of their surface properties and adhesive strength is required. Novel atomic force microscopy experiments conducted under growth conditions (30°C) were used to characterise Escherichia coli biofilms, which were generated by a recently developed spin-coating method onto a poly-l-lysine coated glass substrate. High-resolution topographical images were obtained throughout the course of biofilm development, quantifying the tip–cell interaction force during the 10 day maturation process. Strikingly, the adhesion force between the Si AFM tip and the biofilm surface increased from 0.8nN to 40nN within 3 days. This was most likely due to the production of extracellular polymer substance (EPS), over the maturation period, which was also observed by electron microscopy. At later stages of maturation, multiple retraction events were also identified corresponding to biofilm surface features thought to be EPS components. The spin coated biofilms were shown to have stronger surface adhesion than an equivalent conventionally grown biofilm on the same glass substrate.
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DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Kawasaki,H;Bauerle,R;Zon,G;Ahmed,SA;Miles,EW
通讯作者：
Miles,EW
影响因子：
9.9
作者：
Lorite, Gabriela S.;Rodrigues, Carolina M.;Cotta, Monica A.
通讯作者：
Cotta, Monica A.
影响因子：
13.6
作者：
A. Jesus;F. J. Romano-Baez;L. Leyva-Amezcua;C. Juárez-ramírez;N. Ruiz-Ordaz;J. Galíndez‐Mayer
通讯作者：
A. Jesus;F. J. Romano-Baez;L. Leyva-Amezcua;C. Juárez-ramírez;N. Ruiz-Ordaz;J. Galíndez‐Mayer
影响因子：
2.9
作者：
Sokolov, IY;Firtel, M;Henderson, GS
通讯作者：
Henderson, GS
影响因子：
2.7
作者：
VELIZAROV, SG;RAININA, EI;ZUBOV, AL
通讯作者：
ZUBOV, AL