Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles.
Cell-specific transmembrane injection of molecular cargo with gold nanoparticle-generated transient plasmonic nanobubbles.
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细胞特异性的跨膜注射分子货物用金纳米颗粒产生的瞬态等离子体纳米泡。
DOI：
10.1016/j.biomaterials.2012.03.077
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发表时间：
2012-07
期刊：
影响因子：
14
通讯作者：
Lapotko, Dmitri O.
中科院分区：
文献类型：
--
作者：
Lukianova-Hleb, Ekaterina Y.;Wagner, Daniel S.;Brenner, Malcolm K.;Lapotko, Dmitri O.
最佳的细胞疗法需要高效、选择性和快速地将分子货物运送到靶细胞中，而不损害其生存能力。在体外的异质多细胞系统（如人类移植物）中实现这些目标受到低选择性和货物递送速度以及对靶细胞和非靶细胞的严重损伤的阻碍。我们开发了一种细胞水平的方法，使用瞬态等离子体纳米气泡（PNB）作为细胞特异性纳米注射剂，选择性和引导胞外货物跨膜注射到特定的靶细胞中。作为该方法的技术平台，我们开发了激光流池加工系统。在靶细胞和非靶细胞的异质细胞悬液中测试PNB注射方法和流动体系，将Dextran-FITC染料递送至鳞状细胞癌HN31细胞，并用绿色荧光蛋白编码质粒转染人t细胞。在两种模型中，该方法均表现出单细胞类型选择性、高递送效率（HN31细胞t细胞均为96%）、递送速度（纳秒）和处理后靶细胞的存活率（HN31细胞为96%，t细胞为75%）。因此，PNB注射方法可能有利于实时处理人类移植物，而无需去除生理上重要的细胞。
Optimal cell therapies require efficient, selective and rapid delivery of molecular cargo into target cells without compromising their viability. Achieving these goals ex vivo in bulk heterogeneous multi-cell systems such as human grafts is impeded by low selectivity and speed of cargo delivery and by significant damage to target and non-target cells. We have developed a cell level approach for selective and guided trans-membrane injection of extracellular cargo into specific target cells using transient plasmonic nanobubbles (PNB) as cell-specific nano-injectors. As a technical platform for this method we developed a laser flow cell processing system. The PNB injection method and flow system were tested in heterogeneous cell suspensions of target and non-target cells for delivery of Dextran-FITC dye into squamous cell carcinoma HN31 cells and transfection of human T-cells with a green fluorescent protein-encoding plasmid. In both models the method demonstrated single cell type selectivity, high efficacy of delivery (96% both for HN31 cells T-cells), speed of delivery (nanoseconds) and viability of treated target cells (96% for HN31 cells and 75% for T-cells). The PNB injection method may therefore be beneficial for real time processing of human grafts without removal of physiologically important cells.
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影响因子：
4.3
作者：
Kim, Tae Kyung;Eberwine, James H.
通讯作者：
Eberwine, James H.
影响因子：
3.5
作者：
Hleb, E. Y.;Lapotko, D. O.
通讯作者：
Lapotko, D. O.
影响因子：
10.8
作者：
Anderson, Lindsey J. E.;Hansen, Eric;Lukianova-Hleb, Ekaterina Y.;Hafner, Jason H.;Lapotko, Dmitri O.
通讯作者：
Lapotko, Dmitri O.
影响因子：
2.8
作者：
Choi Y;Yuen C;Maiti SN;Olivares S;Gibbons H;Huls H;Raphael R;Killian TC;Stark DJ;Lee DA;Torikai H;Monticello D;Kelly SS;Kebriaei P;Champlin RE;Biswal SL;Cooper LJ
通讯作者：
Cooper LJ
DOI：
10.1016/j.jconrel.2010.06.004
发表时间：
2010-11-20
期刊：
Journal of controlled release : official journal of the Controlled Release Society
影响因子：
--
作者：
Duncan B;Kim C;Rotello VM
通讯作者：
Rotello VM