A novel system for rapid and cost-effective production of detection and diagnostic reagents of West Nile virus in plants.
A novel system for rapid and cost-effective production of detection and diagnostic reagents of West Nile virus in plants.
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DOI：
10.1155/2012/106783
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发表时间：
2012
影响因子：
--
通讯作者：
Chen Q
中科院分区：
文献类型：
--
作者：
He J;Lai H;Brock C;Chen Q
西尼罗河病毒（WNV）流行的威胁需要开发一个技术平台，可以生产试剂，以支持快速和廉价的检测和诊断。植物表达系统由于其低成本和高可扩展性的性质以及其进行适当的翻译后修饰的能力而对于蛋白质生产是有吸引力的。在这里，我们研究了使用植物生产两种WNV检测和诊断试剂的可行性，以解决当前的成本和可扩展性问题。我们证明了WNV DIII抗原和E16单克隆抗体在两种植物中以高水平快速产生，并且易于纯化。此外，它们在鉴定西尼罗河病毒和检测人对西尼罗河病毒感染的IgM应答中是有效的。E16 mAb与其他黄病毒无交叉反应，对提高诊断准确率有重要价值。本研究为利用植物作为一种稳健、经济的系统生产虫媒病毒诊断试剂提供了原理证明。
The threat of West Nile virus (WNV) epidemics necessitates the development of a technology platform that can produce reagents to support detection and diagnosis rapidly and inexpensively. A plant expression system is attractive for protein production due to its low-cost and high-scalability nature and its ability to make appropriate posttranslational modifications. Here, we investigated the feasibility of using plants to produce two WNV detection and diagnostic reagents to address the current cost and scalability issues. We demonstrated that WNV DIII antigen and E16 monoclonal antibody are rapidly produced at high levels in two plant species and are easily purified. Furthermore, they are effective in identifying WNV and in detecting human IgM response to WNV infection. E16 mAb does not cross-react with other flaviviruses, therefore, is valuable for improving diagnostic accuracy. This study provides a proof of principle for using plants as a robust and economical system to produce diagnostic reagents for arboviruses.
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开发具有针对西尼罗河病毒的治疗潜力的人源化单克隆抗体。
影响因子：
82.9
作者：
Oliphant, Theodore;Engle, Michael;Nybakken, Grant E;Doane, Chris;Johnson, Syd;Huang, Ling;Gorlatov, Sergey;Mehlhop, Erin;Marri, Anantha;Chung, Kyung Min;Ebel, Gregory D;Kramer, Laura D;Fremont, Daved H;Diamond, Michael S
通讯作者：
Diamond, Michael S
影响因子：
4
作者：
Ca単izares, MC;Nicholson, L;Lomonossoff, GP
通讯作者：
Lomonossoff, GP
DOI：
10.1073/pnas.0606631103
发表时间：
2006-10-03
影响因子：
11.1
作者：
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通讯作者：
Gleba, Yuri
影响因子：
5.2
作者：
Negrouk, V;Eisner, G;Wong, HC
通讯作者：
Wong, HC
影响因子：
13.8
作者：
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通讯作者：
Menassa, Rima