Curcumin as a double-edged sword for stem cells: dose, time and cell type-specific responses to curcumin.
Curcumin as a double-edged sword for stem cells: dose, time and cell type-specific responses to curcumin.
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DOI：
10.1186/s40199-015-0115-8
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发表时间：
2015-06-12
期刊：
影响因子：
--
通讯作者：
Hassanzadeh G
中科院分区：
文献类型：
--
作者：
Attari F;Zahmatkesh M;Aligholi H;Mehr SE;Sharifzadeh M;Gorji A;Mokhtari T;Khaksarian M;Hassanzadeh G
姜黄素的有益作用，包括其抗氧化，抗炎和癌症化学预防特性已被确定。关于姜黄素对不同来源干细胞增殖率的最佳剂量和治疗期的信息很少。本研究观察了不同浓度姜黄素对两种优秀干细胞（骨髓干细胞（BMSCs）和成年大鼠神经干/祖细胞（NS/PC））在不同时间点存活和增殖的影响。取成年Wistar大鼠双侧股骨和胫骨分离骨髓间充质干细胞。NS/PC取自成年Wistar大鼠脑室下区。将姜黄素（0.1、0.5、1、5和10 μM/L）加入培养基中培养48或72 h。以5-溴-2 ′-脱氧尿苷（Brdu）阳性细胞的荧光密度作为增殖指标。此外，通过MTT测定来评估细胞活力。48小时后用姜黄素处理BMSC，以剂量依赖性方式增加细胞存活和增殖。姜黄素对神经干细胞的增殖无明显影响，但在10 μM浓度时有毒性作用。72小时的治疗期后，BMSCs和NS/PC存活和增殖与低剂量的姜黄素。然而，高剂量的姜黄素给药72小时显示对两种干细胞的毒性作用。这些发现表明，姜黄素的存活和增殖效应取决于其浓度、治疗时间和干细胞的类型。这些结果的适当应用可能有助于干细胞和姜黄素联合治疗的结果。
The beneficial effects of curcumin which includes its antioxidant, anti-inflammatory and cancer chemo-preventive properties have been identified. Little information is available regarding the optimal dose and treatment periods of curcumin on the proliferation rate of different sources of stem cells. In this study, the effect of various concentrations of curcumin on the survival and proliferation of two types of outstanding stem cells which includes bone marrow stem cells (BMSCs) and adult rat neural stem/progenitor cells (NS/PCs) at different time points was investigated. BMSCs were isolated from bilateral femora and tibias of adult Wistar rats. NS/PCs were obtained from subventricular zone of adult Wistar rat brain. The curcumin (0.1, 0.5, 1, 5 and 10 μM/L) was added into a culture medium for 48 or 72 h. Fluorescent density of 5-bromo-2′-deoxyuridine (Brdu)-positive cells was considered as proliferation index. In addition, cell viability was assessed by MTT assay. Treatment of BMSCs with curcumin after 48 h, increased cell survival and proliferation in a dose-dependent manner. However, it had no effect on NSCs proliferation except a toxic effect in the concentration of 10 μM of curcumin. After a 72 h treatment period, BMSCs and NS/PCs survived and proliferated with low doses of curcumin. However, high doses of curcumin administered for 72 h showed toxic effects on both stem cells. These findings suggest that curcumin survival and proliferative effects depend on its concentration, treatment period and the type of stem cells. Appropriate application of these results may be helpful in the outcome of combination therapy of stem cells and curcumin.
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影响因子：
3.7
作者：
Ormond DR;Shannon C;Oppenheim J;Zeman R;Das K;Murali R;Jhanwar-Uniyal M
通讯作者：
Jhanwar-Uniyal M
影响因子：
1.6
作者：
Son S;Kim KT;Cho DC;Kim HJ;Sung JK;Bae JS
通讯作者：
Bae JS
影响因子：
3
作者：
Aligholi, Hadi;Hassanzadeh, Gholamreza;Gorji, Ali
通讯作者：
Gorji, Ali
影响因子：
4.4
作者：
Al-Omar, Fadhel A.;Nagi, Mahmoud N.;Al-Majed, Abdulhakeem A.
通讯作者：
Al-Majed, Abdulhakeem A.
影响因子：
3.3
作者：
Zbarsky, V;Datla, KP;Dexter, DT
通讯作者：
Dexter, DT