Dehydrogenase genes in the ectomycorrhizal fungus Tricholoma vaccinum: A role for Ald1 in mycorrhizal symbiosis
Dehydrogenase genes in the ectomycorrhizal fungus Tricholoma vaccinum: A role for Ald1 in mycorrhizal symbiosis
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外生菌根真菌痘苗中的脱氢酶基因:Ald1 在菌根共生中的作用
DOI:
10.1002/jobm.201500381
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发表时间:
2015
影响因子:
3.1
通讯作者:
Krause K
中科院分区:
文献类型:
--
作者:
Henke C;Jung E-M;Voit A;Kothe E;Krause K
Ectomycorrhizal symbiosis is important for forest ecosystem functioning with tree‐fungal cooperation increasing performance and countering stress conditions. Aldehyde dehydrogenases (ALDHs) are key enzymes for detoxification and thus may play a role in stress response of the symbiotic association. With this focus, eight dehydrogenases, Ald1 through Ald7 and TyrA, of the ectomycorrhizal basidiomyceteTricholoma vaccinumwere characterized and phylogenetically investigated. Functional analysis was performed through differential expression analysis by feeding different, environmentally important substances. A strong effect of indole‐3‐acetic acid (IAA) was identified, linking mycorrhiza formation and auxin signaling between the symbiosis partners. We investigatedald1overexpressing strains for performance in mycorrhiza with the host tree spruce (Picea abies) and observed an increased width of the apoplast, accommodating the Hartig’ net hyphae of theT. vaccinumover‐expressing transformants. The results support a role for Ald1 in ectomycorrhiza formation and underline functional differentiation within fungal aldehyde dehydrogenases in the family 1 of ALDHs.
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影响因子:
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通讯作者:
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