Dehydrogenase genes in the ectomycorrhizal fungus Tricholoma vaccinum: A role for Ald1 in mycorrhizal symbiosis

Dehydrogenase genes in the ectomycorrhizal fungus Tricholoma vaccinum: A role for Ald1 in mycorrhizal symbiosis
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外生菌根真菌痘苗中的脱氢酶基因:Ald1 在菌根共生中的作用

DOI:
10.1002/jobm.201500381
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发表时间:
2015
影响因子:
3.1
通讯作者:
Krause K
Krause K
中科院分区:
生物学4区
文献类型:
--
作者:
Henke C;Jung E-M;Voit A;Kothe E;Krause K

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外生菌根共生对于森林生态系统功能具有重要意义,树木-真菌合作提高性能并对抗胁迫条件。乙醛脱氢酶(ALDHs)是植物解毒的关键酶,可能在共生体的应激反应中发挥重要作用。因此,我们对外生菌根菌口蘑的八种内切酶Ald 1 ~ Ald 7和TyrA进行了表征和遗传学研究。通过饲喂不同的环境重要物质,通过差异表达分析进行功能分析。吲哚-3-乙酸(IAA)的强烈作用被确定,将共生伙伴之间的菌根形成和生长素信号传导联系起来。我们研究了11个过表达菌株在云杉(Picea abies)的菌根中的表现,观察到质外体的宽度增加,可容纳T.表达vaccinumor的转化体。这些结果支持了Ald 1在外生菌根形成中的作用,并强调了ALDH家族1中真菌乙醛脱氢酶的功能分化。
Ectomycorrhizal symbiosis is important for forest ecosystem functioning with tree‐fungal cooperation increasing performance and countering stress conditions. Aldehyde dehydrogenases (ALDHs) are key enzymes for detoxification and thus may play a role in stress response of the symbiotic association. With this focus, eight dehydrogenases, Ald1 through Ald7 and TyrA, of the ectomycorrhizal basidiomyceteTricholoma vaccinumwere characterized and phylogenetically investigated. Functional analysis was performed through differential expression analysis by feeding different, environmentally important substances. A strong effect of indole‐3‐acetic acid (IAA) was identified, linking mycorrhiza formation and auxin signaling between the symbiosis partners. We investigatedald1overexpressing strains for performance in mycorrhiza with the host tree spruce (Picea abies) and observed an increased width of the apoplast, accommodating the Hartig’ net hyphae of theT. vaccinumover‐expressing transformants. The results support a role for Ald1 in ectomycorrhiza formation and underline functional differentiation within fungal aldehyde dehydrogenases in the family 1 of ALDHs.
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