Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometry.
Rapid and sensitive detection of SARS-CoV-2 antibodies by biolayer interferometry.
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用生物层干涉法快速灵敏地检测SARS-CoV-2抗体
DOI：
10.1038/s41598-020-78895-x
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发表时间：
2020-12-10
影响因子：
4.6
通讯作者：
DuBois RM
中科院分区：
文献类型：
--
作者：
Dzimianski JV;Lorig-Roach N;O'Rourke SM;Alexander DL;Kimmey JM;DuBois RM
评估血浆中抗原特异性抗体的血清学检测通常通过缺乏定量结果的快速横向流动试纸或通过耗时费力但提供半定量结果的高复杂性免疫测定来进行。在这里，我们描述了一种新的应用生物层干涉法快速检测血浆样品中抗原特异性抗体水平，并证明了其用于定量SARS-CoV-2抗体的实用性。我们的生物层干涉免疫吸附测定（bbi - isa）采用一次性生物传感器自动“浸读”格式，提供抗原负载、血浆抗体结合和抗体同型检测的实时光学测量。在不到20分钟的时间内获得了完整的半定量结果。BLI-ISA达到或超过高复杂性方法的性能，如酶联免疫吸附测定（ELISA）和化学发光免疫测定。重要的是，我们的方法可以立即在现有的BLI平台上实施，用于COVID-19紧急研究，如血清监测和候选疫苗评估。从更广泛的意义上说，BLI-ISA可以作为一种新的诊断平台来评估临床标本中的抗体和其他生物分子。
Serological testing to evaluate antigen-specific antibodies in plasma is generally performed by rapid lateral flow test strips that lack quantitative results or by high complexity immunoassays that are time- and labor-intensive but provide semi-quantitative results. Here, we describe a novel application of biolayer interferometry for the rapid detection of antigen-specific antibody levels in plasma samples, and demonstrate its utility for quantification of SARS-CoV-2 antibodies. Our biolayer interferometry immunosorbent assay (BLI-ISA) utilizes single-use biosensors in an automated “dip-and-read” format, providing real-time optical measurements of antigen loading, plasma antibody binding, and antibody isotype detection. Complete semi-quantitative results are obtained in less than 20 min. BLI-ISA meets or exceeds the performance of high complexity methods such as Enzyme-Linked Immunosorbent Assay (ELISA) and Chemiluminescent Immunoassay. Importantly, our method can be immediately implemented on existing BLI platforms for urgent COVID-19 studies, such as serosurveillance and the evaluation of vaccine candidates. In a broader sense, BLI-ISA can be developed as a novel diagnostic platform to evaluate antibodies and other biomolecules in clinical specimens.
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影响因子：
4.1
作者：
Herrera NG;Morano NC;Celikgil A;Georgiev GI;Malonis RJ;Lee JH;Tong K;Vergnolle O;Massimi AB;Yen LY;Noble AJ;Kopylov M;Bonanno JB;Garrett-Thomson SC;Hayes DB;Bortz RH 3rd;Wirchnianski AS;Florez C;Laudermilch E;Haslwanter D;Fels JM;Dieterle ME;Jangra RK;Barnhill J;Mengotto A;Kimmel D;Daily JP;Pirofski LA;Chandran K;Brenowitz M;Garforth SJ;Eng ET;Lai JR;Almo SC
通讯作者：
Almo SC
影响因子：
28.3
作者：
Gorbalenya, Alexander E.;Baker, Susan C.;Ziebuhr, John
通讯作者：
Ziebuhr, John
影响因子：
8.4
作者：
Auer, Sanna;Koho, Tiia;Hytonen, Vesa P.
通讯作者：
Hytonen, Vesa P.
影响因子：
82.9
作者：
Long, Quan-Xin;Liu, Bai-Zhong;Huang, Ai-Long
通讯作者：
Huang, Ai-Long
影响因子：
13.2
作者：
Chan, Jasper Fuk-Woo;Kok, Kin-Hang;Yuen, Kwok-Yung
通讯作者：
Yuen, Kwok-Yung