Polymorphisms of the IL-17A Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows.
Polymorphisms of the IL-17A Gene Influence Milk Production Traits and Somatic Cell Score in Chinese Holstein Cows.
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DOI：
10.3390/bioengineering9090448
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发表时间：
2022-09-07
影响因子：
4.6
通讯作者：
Mao, Yongjiang
中科院分区：
文献类型：
--
作者：
Mohyuddin, Sahar Ghulam;Liang, Yan;Ni, Wei;Arbab, Abdelaziz Adam Idriss;Zhang, Huiming;Li, Mingxun;Yang, Zhangping;Karrow, Niel A.;Mao, Yongjiang
奶牛的产奶特性是畜牧业中一个重要的经济指标。血清细胞因子如白细胞介素17（IL-17）可能是奶牛乳房炎的潜在指标，与乳汁体细胞计数（SCC）和体细胞评分（SCS）有关。本研究旨在发现牛IL-17 A基因的单核苷酸多态性，并进一步研究其与中国荷斯坦奶牛产奶性状的关系。从江苏省6个奶牛场随机选取20头中国荷斯坦奶牛。从选定的血液样品中提取DNA用于PCR扩增，序列分析用于发现牛（IL-17 A）基因中的SNPs。在5′UTR区发现的5个SNPs分别为g-1578 A>G、g-1835 G>A和g-398 T>A，外显子区为g3164 T>C和g3409 G>C。基于Sequenom Mass ARRAY和SNP数据对荷斯坦奶牛（n = 992）进行基因分型。使用最小二乘法研究SNP、产奶量变量和体细胞评分之间的联系。结果表明，SNP g-398 T>A与g3164 T>C存在显著的连锁不平衡。发现SNPs与试验日产奶量具有显著（p < 0.05）相关性。综上所述，IL-17 A显著影响奶牛的产奶性状。
The cow’s milk production characteristics are a significant economic indicator in the livestock industry. Serum cytokines such as interleukin-17 (IL-17) may be potential indicators for bovine mastitis concerning the milk somatic cell count (SCC) and somatic cell score (SCS). The current study aims to find previously undiscovered single nucleotide polymorphisms in the bovine (IL-17A) gene and further investigates their associations with milk production traits in Chinese Holstein cows. Twenty Chinese Holstein cows were randomly chosen from six farms in Jiangsu Province, China. The DNA was extracted from selected samples of bloods for PCR amplification Sequence analyses were used to find SNPs in the bovine (IL-17A) gene. The discovered five SNPs are g-1578A>G, g-1835G>A, and g-398T>A in the 5′UTR; g3164T>C and g3409G>C in the exon region. The genotyping of Holstein cows (n = 992) was performed based on Sequenom Mass ARRAY and SNP data. The connection between SNPs, milk production variables, and the somatic cell score was investigated using the least-squares method. Based on the results, SNP g-398T>A had a significant linkage disequilibrium with g3164T>C. SNPs were found to have significant (p < 0.05) correlations with the test-day milk yield. In conclusion, IL-17A affects cow’s milk production traits significantly.
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影响因子：
4.4
作者：
Gilbert FB;Cunha P;Jensen K;Glass EJ;Foucras G;Robert-Granié C;Rupp R;Rainard P
通讯作者：
Rainard P
影响因子：
5.4
作者：
HILL W G;ROBERTSON A
通讯作者：
ROBERTSON A
影响因子：
4.5
作者：
Cvetnić L;Samardžija M;Duvnjak S;Habrun B;Cvetnić M;Jaki Tkalec V;Đuričić D;Benić M
通讯作者：
Benić M
影响因子：
3.5
作者：
Oliveira, H. R.;Cant, J. P.;Schenkel, F. S.
通讯作者：
Schenkel, F. S.
影响因子：
3.8
作者：
Bougarn, Salim;Cunha, Patricia;Rainard, Pascal
通讯作者：
Rainard, Pascal