Multi Locus Sequence Typing and spa Typing of Staphylococcus Aureus Isolated from the Milk of Cows with Subclinical Mastitis in Croatia.
Multi Locus Sequence Typing and spa Typing of Staphylococcus Aureus Isolated from the Milk of Cows with Subclinical Mastitis in Croatia.
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DOI：
10.3390/microorganisms9040725
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发表时间：
2021-03-31
期刊：
影响因子：
4.5
通讯作者：
Benić M
中科院分区：
文献类型：
--
作者：
Cvetnić L;Samardžija M;Duvnjak S;Habrun B;Cvetnić M;Jaki Tkalec V;Đuričić D;Benić M
背景：在大多数奶业国家，金黄色葡萄球菌是引起奶牛乳房炎的最常见的病原体。金黄色葡萄球菌的感染率从2%到50%以上不等，占临床乳房炎病例的10%-12%。目的：对从奶牛隐性乳房炎乳汁中分离的237株金黄色葡萄球菌进行spA、mecA、mecC和pvl基因分析，并进行spA和多位点序列分型。方法：对扩增出的基因序列在Macrogen Europe进行测序。用Ridom StaphType和BioNumerics软件对获得的spA和7个看家基因的序列进行分析。结果：204株(86.1%)检测到spA基因，10株检测到mecA和mecC基因，未检测到pvl基因。SpA分型成功分析了153株测试菌株(64.3%)，确认了53个SpA类型，其中4个为新类型。最常见的温泉类型为t2678(14%)。MLST分型198株(83.5%)，确定了32种不同的等位基因图谱，其中3种为新等位基因。最常见的等位基因是ST133(20.7%)。定义了6个组(G)和15个独生子女。结论：从隐性乳房炎奶牛乳汁中分离出的金黄色葡萄球菌属于异质性群，考虑到确诊的SpA类型和序列类型(STS)。为了检查分离株可能的人畜共患病潜力，有必要对农场上的人和其他牲畜进行检测。
Background: The bacterial species S. aureus is the most common causative agent of mastitis in cows in most countries with a dairy industry. The prevalence of infection caused by S. aureus ranges from 2% to more than 50%, and it causes 10–12% of all cases of clinical mastitis. Aim: The objective was to analyze 237 strains of S. aureus isolated from the milk of cows with subclinical mastitis regarding the spa, mecA, mecC and pvl genes and to perform spa and multi-locus sequence typing (MLST). Methods: Sequencing amplified gene sequences was conducted at Macrogen Europe. Ridom StaphType and BioNumerics software was used to analyze obtained sequences of spa and seven housekeeping genes. Results: The spa fragment was present in 204 (86.1%) of strains, while mecA and mecC gene were detected in 10 strains, and the pvl gene was not detected. Spa typing successfully analyzed 153 tested isolates (64.3%), confirming 53 spa types, four of which were new types. The most frequent spa type was t2678 (14%). MLST typed 198 (83.5%) tested strains and defined 32 different allele profiles, of which three were new. The most frequent allele profile was ST133 (20.7%). Six groups (G) and 15 singletons were defined. Conclusion: Taking the number of confirmed spa types and sequence types (STs) into account, it can be concluded that the strains of S. aureus isolated from the milk of cows with subclinical mastitis form a heterogenous group. To check the possible zoonotic potential of isolates it would be necessary to test the persons and other livestock on the farms.
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影响因子：
5.7
作者：
Li T;Lu H;Wang X;Gao Q;Dai Y;Shang J;Li M
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