Genome analysis and expression patterns of odorant-binding proteins from the Southern House mosquito Culex pipiens quinquefasciatus.
Genome analysis and expression patterns of odorant-binding proteins from the Southern House mosquito Culex pipiens quinquefasciatus.
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DOI：
10.1371/journal.pone.0006237
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发表时间：
2009-07-16
期刊：
影响因子：
3.7
通讯作者：
Leal WS
中科院分区：
文献类型：
--
作者：
Pelletier J;Leal WS
蚊子的嗅觉行为是由气味结合蛋白（OBP）介导的。它们形成了一个多基因家族，参与昆虫嗅觉的外周事件，特别是将气味物质转运到膜结合的气味受体。OBP有助于昆虫嗅觉系统的显着敏感性，并可能参与气味物质的选择性运输。我们采用了生物信息学和分子生物学方法相结合的方法来识别和表征南方库蚊致倦库蚊（= Cx）中“经典”OBP家族的成员。 quinquefasciatus），一种引起多种人类疾病的病原体载体。通过利用最近公布的基因组序列，我们已经确定了53个推定的Cx。通过Blast搜索致倦犬OBP基因。作为对它们的分子表征的第一步，通过RT-PCR的表达模式揭示了13个基因，这些基因在化学感受组织中被专门和大量地检测到。使用半定量RT-PCR，没有观察到明显的差异，嗅觉特异性OBP的转录水平之间的触角的性别。系统发育分析和比较分析表明Cx是同源的。冈比亚按蚊和埃及伊蚊致倦按蚊OBP。53个推定的OBP基因在Cx中的鉴定。quinquefasciatus突出了这个家庭的多样性。组织特异性研究表明，在蚊子OBP家族中存在不同的功能类别。大多数基因在化学感受器以及非化学感受器组织中检测到，表明它们可能是嗅觉蛋白，但不一定是嗅觉蛋白。另一方面，13个“真正的”OBP基因只在嗅觉组织中被检测到，并可能专门参与检测“关键”的信息化学物质。有趣的是，在CX。quinquefasciatus嗅觉特异性OBP仅属于四个不同的系统发育组，这些组在三种蚊子物种中特别保守。
Olfactory-based behaviors in mosquitoes are mediated by odorant-binding proteins (OBPs). They form a multigenic family involved in the peripheral events in insect olfaction, specifically the transport of odorants to membrane-bound odorant receptors. OBPs contribute to the remarkable sensitivity of the insect's olfactory system and may be involved in the selective transport of odorants. We have employed a combination of bioinformatics and molecular approaches to identify and characterize members of the “classic” OBP family in the Southern House mosquito Culex pipiens quinquefasciatus ( = Cx. quinquefasciatus), a vector of pathogens causing several human diseases. By taking advantage of the recently released genome sequences, we have identified fifty-three putative Cx. quinquefasciatus OBP genes by Blast searches. As a first step towards their molecular characterization, expression patterns by RT-PCR revealed thirteen genes that were detected exclusively and abundantly in chemosensory tissues. No clear differences were observed in the transcripts levels of olfactory-specific OBPs between antennae of both sexes using semi-quantitative RT-PCR. Phylogenetic and comparative analysis revealed orthologous of Cx. quinquefasciatus OBPs in Anopheles gambiae and Aedes aegypti. The identification of fifty-three putative OBP genes in Cx. quinquefasciatus highlights the diversity of this family. Tissue-specificity study suggests the existence of different functional classes within the mosquito OBP family. Most genes were detected in chemosensory as well as non chemosensory tissues indicating that they might be encapsulins, but not necessarily olfactory proteins. On the other hand, thirteen “true” OBP genes were detected exclusively in olfactory tissues and might be involved specifically in the detection of “key” semiochemicals. Interestingly, in Cx. quinquefasciatus olfactory-specific OBPs belong exclusively to four distinct phylogenetic groups which are particularly well conserved among three mosquito species.
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DOI：
10.1073/pnas.0501447102
发表时间：
2005-04-12
影响因子：
11.1
作者：
Leal, WS;Chen, AM;Tsuruda, JM
通讯作者：
Tsuruda, JM
影响因子：
2.2
作者：
Li, ZX;Pickett, JA;Zhou, JJ
通讯作者：
Zhou, JJ
DOI：
10.1016/j.bbrc.2005.07.176
发表时间：
2005-10-07
影响因子：
3.1
作者：
Lautenschlager, C;Leal, WS;Clardy, J
通讯作者：
Clardy, J
影响因子：
7
作者：
Hekmat-Scafe, DS;Scafe, CR;Tanouye, MA
通讯作者：
Tanouye, MA
影响因子：
3.7
作者：
Leal WS;Barbosa RM;Xu W;Ishida Y;Syed Z;Latte N;Chen AM;Morgan TI;Cornel AJ;Furtado A
通讯作者：
Furtado A