High-Throughput Electrochemical Microfluidic Immunoarray for Multiplexed Detection of Cancer Biomarker Proteins.
High-Throughput Electrochemical Microfluidic Immunoarray for Multiplexed Detection of Cancer Biomarker Proteins.
复制标题
DOI：
10.1021/acssensors.6b00256
复制
发表时间：
2016-08-26
期刊：
影响因子：
8.9
通讯作者：
Rusling, James F.
中科院分区：
文献类型：
--
作者：
Tang, Chi K.;Vaze, Abhay;Shen, Min;Rusling, James F.
基于微芯片的微流控电化学阵列对于使用相对简单的仪器以每次测定的低成本快速、高通量多重检测癌症生物标志物蛋白具有很大的希望。在这里，我们描述了一种廉价的高通量电化学阵列，其特征在于32个可单独寻址的微电极，其进一步与8端口歧管复用以提供256个传感器。金电极阵列是通过湿法蚀刻商业金光盘（CD-R），然后图案化绝缘。使用印刷-剥离方法在每个传感器周围产生亚微升疏水威尔斯孔，以消除捕获抗体固定期间的交叉污染。使用连接到小型化8端口歧管的8个32传感器免疫阵列实现了高通量分析，允许在<1 h内进行256次测量。该系统用于测定血清中的前列腺癌生物标志物蛋白前列腺特异性抗原（PSA）、前列腺特异性膜抗原（PSMA）、白细胞介素-6（IL-6）和血小板因子-4（PF-4）。利用在修饰有酶标记和抗体的磁性纳米颗粒上预捕获分析物蛋白质，这些蛋白质达到了临床相关的检测限（0.05至2 pg mL−1）和5-十倍动态范围（低于pg mL−1至远高于ng mL−1）。
Microchip-based microfluidic electrochemical arrays hold great promise for fast, high-throughput multiplexed detection of cancer biomarker proteins at low cost per assay using relatively simple instrumentation. Here we describe an inexpensive high-throughput electrochemical array featuring 32 individually addressable microelectrodes that is further multiplexed with an 8-port manifold to provide 256 sensors. The gold electrode arrays were fabricated by wet-etching commercial gold compact discs (CD-R) followed by patterned insulation. A print-and-peel method was used to create sub-microliter hydrophobic wells surrounding each sensor to eliminate cross contamination during immobilization of capture antibodies. High-throughput analyses were realized using eight 32-sensor immunoarrays connected to the miniaturized 8-port manifold, allowing 256 measurements in <1 h. This system was used to determine prostate cancer biomarker proteins prostate specific antigen (PSA), prostate specific membrane antigen (PSMA), interleukin-6 (IL-6), and platelet factor-4 (PF-4) in serum. Clinically relevant detection limits (0.05 to 2 pg mL−1) and 5-decade dynamic ranges (sub pg mL−1 to well above ng mL−1) were achieved for these proteins utilizing precapture of analyte proteins on magnetic nanoparticles decorated with enzyme labels and antibodies.
登录
查看更多内容
影响因子：
12.6
作者：
Chikkaveeraiah, Bhaskara V.;Mani, Vigneshwaran;Patel, Vyomesh;Gutkind, J. Silvio;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
9.9
作者：
Jeyachandran, Y. L.;Mielczarski, J. A.;Rai, B.
通讯作者：
Rai, B.
影响因子：
7.4
作者：
Mani, Vigneshwaran;Wasalathanthri, Dhanuka P.;Joshi, Arnit A.;Kumar, Challa V.;Rusling, James F.
通讯作者：
Rusling, James F.
影响因子：
16.6
作者：
Lam, Brian;Das, Jagotamoy;Kelley, Shana O.
通讯作者：
Kelley, Shana O.
血清促炎细胞因子和C反应蛋白的超敏感性微流体阵列评估癌症患者口腔粘膜炎的风险。
影响因子：
4.3
作者：
Krause CE;Otieno BA;Bishop GW;Phadke G;Choquette L;Lalla RV;Peterson DE;Rusling JF
通讯作者：
Rusling JF