Effect of osmotic protection on nucleated cell killing by C5b-9: cell death is not affected by the prevention of cell swelling.
Effect of osmotic protection on nucleated cell killing by C5b-9: cell death is not affected by the prevention of cell swelling.
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渗透保护对 C5b-9 杀伤有核细胞的影响：细胞死亡不受细胞肿胀预防的影响。
DOI：
10.1016/0161-5890(89)90087-4
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发表时间：
1989
影响因子：
3.6
通讯作者：
Shin,ML
中科院分区：
文献类型：
--
作者：
Kim,SH;Carney,DF;Papadimitriou,JC;Shin,ML
质膜中C5 b-9通道的形成可导致红细胞溶解或有核细胞死亡。由于离子和水通过C5 b-9通道的流量不受调节，胶体渗透肿胀和质膜破裂导致红细胞被补体溶解。这种胶体渗透溶解机制主要基于以下证据：当将大分子置于培养基中以平衡细胞内大分子产生的渗透梯度时，溶血程度降低，细胞内大分子太大而不能通过补体通道扩散。然而，胶体渗透调节失调作为C5 b-9杀死有核细胞的原因的作用最近受到质疑[Kim S.，卡尼和Shin M.L.J.Immun.138，1530（1987）]。在本研究中，我们研究了81，000 mol. wt右旋糖酐或牛血清白蛋白的渗透保护作用对补体通道杀伤埃利希细胞的影响。结果表明，当通过活体染料染色评估时，葡聚糖对细胞肿胀的预防不会降低小（C5 b-91）或大（C5 b-9 m）补体通道对有核细胞杀伤的程度或速率。然而，在葡聚糖或白蛋白的存在下，在防止细胞肿胀的浓度下，细胞质乳酸脱氢酶的释放作为细胞死亡的替代措施被延迟和/或减少。这些结果表明，C5 b-9可以有效地杀死有核细胞，在胶体渗透细胞肿胀的情况下，和细胞质大分子的释放可能不是一个可靠的指标细胞死亡时，渗透保护剂。
Formation of C5b-9 channels in the plasma membrane can lead to erythrocyte lysis or nucleated cell death. Lysis of erythrocytes by complement occurs as a result of colloid osmotic swelling and rupture of the plasma membrane, due to the unregulated flux of ions and water through C5b-9 channels. This colloid osmotic mechanism of lysis is largely based on the evidence that the extent of hemolysis is reduced, when macromolecules are placed in the medium to balance the osmotic gradient created by intracellular macromolecules, which are too large to diffuse through complement channels. The role of colloid osmotic deregulation, as a cause of nucleated cell killing by C5b-9, however, has been recently questioned [Kim S., Carney D.F. and Shin M.L.J. Immun.138, 1530 (1987)]. In the present study, we investigated the effect of osmotic protection, with an 81,000mol.wt dextran or bovine serum albumin, on Ehrlich cell killing by complement channels. The results indicated that prevention of cell swelling by dextran did not reduce the extent or rate of nucleated cell killing by either small (C5b-91), or large (C5b-9m), complement channels when assessed by vital dye stain. The release of cytoplasmic lactate dehydrogenase as an alternative measure of cell death, however, was retarded and/or reduced, in the presence of dextran or albumin, at concns that prevented cell swelling. These results indicate that C5b-9 can kill nucleated cells effectively, in the absence of colloidal osmotic cell swelling, and that release of cytoplasmic macromolecules may not be a reliable indicator of cell death, when osmotic protectants are employed.
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补体攻击巨噬细胞后花生四烯酸的释放和氧化衍生物的形成：通道形成的作用。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Imagawa,DK;Osifchin,NE;Ramm,LE;Koga,PG;Hammer,CH;Shin,HS;Mayer,MM
通讯作者：
Mayer,MM
末端补体复合物水解人髓磷脂中的髓磷脂碱性蛋白。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Vanguri,P;Shin,ML
通讯作者：
Shin,ML
影响因子：
4.4
作者：
Michael M. Frank;Herbert J. Rapp;Tibor Borsos
通讯作者：
Tibor Borsos
大剂量补体形成的跨膜通道的寿命和大小。
影响因子：
4.4
作者：
L. Ramm;M. Mayer
通讯作者：
M. Mayer
影响因子：
4.4
作者：
Michael Boyle;Tibor Borsos
通讯作者：
Tibor Borsos