Tension-compression loading with chemical stimulation results in additive increases to functional properties of anatomic meniscal constructs.
Tension-compression loading with chemical stimulation results in additive increases to functional properties of anatomic meniscal constructs.
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DOI：
10.1371/journal.pone.0027857
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Athanasiou KA
中科院分区：
文献类型：
--
作者：
Huey DJ;Athanasiou KA
本研究旨在通过同时拉伸和压缩机械刺激结合化学刺激来改善解剖形状半月板结构的功能特性。对无支架的髋臼结构进行同时的拉伸和压缩刺激以及化学刺激。机械负荷的时间方面进行了研究，采用两个独立的五天刺激期。化学刺激包括应用分解代谢GAG消耗酶软骨素酶ABC（C-ABC）和合成代谢生长因子TGF-β1。通过全因子实验设计研究了机械和化学刺激组合，并在培养4周后评估了组织学、生物化学和生物力学特性。从第10-14天施加的机械负荷导致骨组织结构的压缩、拉伸和生物化学性质显著增加。当机械和化学stimuliwere相结合的显着添加剂增加胶原蛋白每湿重（4倍），压缩瞬时（3倍）和松弛（2倍）模量，并在圆周（4倍）和径向（6倍）方向的拉伸模量。本研究表明，同时进行拉伸和压缩机械刺激、C-ABC和TGF-β1的刺激方案能够创建解剖半月板结构，复制天然组织的压缩机械特性以及胶原和GAG含量。此外，这项研究显着推进半月板组织工程，是第一个同时施加拉伸和压缩机械刺激，并观察机械刺激后的拉伸和压缩性能的增强。
This study aimed to improve the functional properties of anatomically-shaped meniscus constructs through simultaneous tension and compression mechanical stimulation in conjunction with chemical stimulation. Scaffoldless meniscal constructs were subjected to simultaneous tension and compressive stimulation and chemical stimulation. The temporal aspect of mechanical loadingwas studied by employing two separate five day stimulation periods. Chemical stimulation consisted of the application of a catabolic GAG-depleting enzyme, chondroitinase ABC (C-ABC), and an anabolic growth factor, TGF-β1. Mechanical and chemical stimulation combinations were studied through a full-factorial experimental design and assessed for histological, biochemical, and biomechanical properties following 4 wks of culture. Mechanical loading applied from days 10–14 resulted in significant increases in compressive, tensile, and biochemical properties of meniscal constructs. When mechanical and chemical stimuliwere combined significant additive increases in collagen per wet weight (4-fold), compressive instantaneous (3-fold) and relaxation (2-fold) moduli, and tensile moduli in the circumferential (4-fold) and radial (6-fold) directions were obtained. This study demonstrates that a stimulation regimen of simultaneous tension and compression mechanical stimulation, C-ABC, and TGF-β1 is able to create anatomic meniscus constructs replicating the compressive mechanical properties, and collagen and GAG content of native tissue. In addition, this study significantly advances meniscus tissue engineering by being the first to apply simultaneous tension and compression mechanical stimulation and observe enhancement of tensile and compressive properties following mechanical stimulation.
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影响因子：
14
作者：
Gunja, Najmuddin J.;Uthamanthil, Rajesh K.;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Athanasiou, Kyriacos A.
影响因子：
7
作者：
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通讯作者：
Levenston, ME