Effects of TGF-beta1 and hydrostatic pressure on meniscus cell-seeded scaffolds.
Effects of TGF-beta1 and hydrostatic pressure on meniscus cell-seeded scaffolds.
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DOI：
10.1016/j.biomaterials.2008.10.007
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发表时间：
2009-02
期刊：
影响因子：
14
通讯作者：
Athanasiou, Kyriacos A.
中科院分区：
文献类型：
--
作者：
Gunja, Najmuddin J.;Uthamanthil, Rajesh K.;Athanasiou, Kyriacos A.
采用两阶段序贯研究，研究了TGF-β1和静水压力（HP）对半月板细胞接种PLLA构建体的组合作用。我们的目标是确定潜在的协同效应，这些刺激对提高生物力学和组成特征的工程结构。在I期，检查TGF-β1对半月板细胞产生ECM的能力的影响。在阶段II中，将半月板细胞接种的PLLA构建体与TGF-β1和HP（10 MPa，0 Hz或10 MPa，0.1 Hz）的组合培养4周。在I期，发现TGF-β1使支架中的胶原和GAG沉积分别增加15倍和8倍。在阶段II中，当与未加压的无生长因子培养物对照相比时，TGF-β1和10 MPa、0 Hz HP的组合导致4倍更高的胶原沉积（累加增加）、3倍更高的GAG沉积和增强的压缩特性（累加和协同增加）。尽管在压缩特性（模量和粘度）与结构的GAG和胶原含量之间观察到显著的相关性，但是与胶原的相关性更强。本研究提供了强有力的证据，证明生长因子和HP可以成功地联合使用，以增强体外工程化膝关节半月板结构的功能特性。
The combinatorial effects of TGF-β1 and hydrostatic pressure (HP) were investigated on meniscus cell-seeded PLLA constructs using a two-phase sequential study. The objective was to identify potentially synergistic effects of these stimuli toward enhancing the biomechanical and compositional characteristics of the engineered constructs. In Phase I, the effects of TGF-β1 were examined on the ability of meniscus cells to produce ECM. In Phase II, meniscus cell-seeded PLLA constructs were cultured for 4 wks with a combination of TGF-β1 and HP (10 MPa, 0 Hz or 10 MPa, 0.1 Hz). TGF-β1 was found to increase collagen and GAG deposition in the scaffolds 15-fold and 8-fold, respectively, in Phase I. In Phase II, the combination of TGF-β1 and 10 MPa, 0 Hz HP resulted in 4-fold higher collagen deposition (additive increase), 3-fold higher GAG deposition and enhanced compressive properties (additive and synergistic increases), when compared to the unpressurized no growth factor culture control. Though significant correlations were observed between the compressive properties (moduli and viscosity), and the GAG and collagen content of the constructs, the correlations were stronger with collagen. This study provides robust evidence that growth factors and HP can be used successfully in combination to enhance the functional properties of in vitro engineered knee meniscus constructs.
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影响因子：
7
作者：
Imler, SM;Doshi, AN;Levenston, ME
通讯作者：
Levenston, ME
影响因子：
7.6
作者：
Allen, K. D.;Athanasiou, K. A.
通讯作者：
Athanasiou, K. A.
影响因子：
3.8
作者：
Almarza, AJ;Athanasiou, KA
通讯作者：
Athanasiou, KA
DOI：
10.3109/17453679909000959
发表时间：
1999-02-01
期刊：
ACTA ORTHOPAEDICA SCANDINAVICA
影响因子：
--
作者：
de Haart, M;Marijnissen, WJCM;Verhaar, JAN
通讯作者：
Verhaar, JAN
影响因子：
7
作者：
Marsano, A.;Millward-Sadler, S. J.;Martin, I.
通讯作者：
Martin, I.