A novel electrospun polylactic acid silkworm fibroin mesh for abdominal wall hernia repair.
A novel electrospun polylactic acid silkworm fibroin mesh for abdominal wall hernia repair.
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DOI：
10.1016/j.mtbio.2023.100915
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发表时间：
2024-02
影响因子：
8.2
通讯作者：
Wei, Guangbing
中科院分区：
文献类型：
--
作者：
Wang, Xingjie;Liu, Changjun;Li, Xuqi;Shen, Tianli;Lian, Jie;Shi, Jing;Jiang, Zhengdong;Qiu, Guanglin;Wang, Yuanbo;Meng, Er;Wei, Guangbing
腹壁疝是常见的腹部疾病，有效的疝修补术具有挑战性。在临床实践中，合成补片被广泛用于修补腹壁疝。然而，术后并发症，如炎症和粘连，是普遍的。生物补片虽然可以在一定程度上解决这一问题，但面临着材料不均匀、降解速度快、力学性能一般、成本高等问题。在这里，制造了一种由聚乳酸和丝素蛋白（PLA-SF）组成的新型静电纺丝补片，用于修复腹壁疝，具有良好的物理性能，生物相容性和低生产成本。FTIR和EDS用于证明PLA-SF网片成功合成。通过溶胀实验和体外降解实验对PLA-SF的理化性质进行了检测。水接触角反映了材料的亲水性，应力-应变曲线反映了材料的力学性能。建立大鼠腹壁疝模型，观察体内降解、粘连和炎症。体外细胞培养实验用于检测细胞相容性和寻找受影响的生化途径。PLA-SF补片成功合成，并且在体外不会随时间膨胀或降解。它具有很高的亲水性和强度。在大鼠模型中，PLA-SF补片显著减少腹部炎症并抑制粘连形成。PLA-SF补片的体外降解速率比组织重塑慢。共培养实验表明，PLA-SF补片通过TGF-β1/Smad通路降低了成纤维细胞分泌的炎性因子的表达，并促进了成纤维细胞增殖。PLA-SF补片具有良好的理化性能和生物相容性，促进大鼠腹壁疝修补，减少术后炎症和粘连。这是一种很有前途的补片，具有临床应用潜力。
Abdominal wall hernias are common abdominal diseases, and effective hernia repair is challenging. In clinical practice, synthetic meshes are widely applied for repairing abdominal wall hernias. However, postoperative complications, such as inflammation and adhesion, are prevalent. Although biological meshes can solve this problem to a certain extent, they face the problems of heterogeneity, rapid degradation rate, ordinary mechanical properties, and high-cost. Here, a novel electrospinning mesh composed of polylactic acid and silk fibroin (PLA-SF) for repairing abdominal wall hernias was manufactured with good physical properties, biocompatibility and low production cost. FTIR and EDS were used to demonstrate that the PLA-SF mesh was successfully synthesized. The physicochemical properties of PLA-SF were detected by swelling experiments and in vitro degradation experiments. The water contact angle reflected the hydrophilicity, and the stress‒strain curve reflected the mechanical properties. A rat abdominal wall hernia model was established to observe degradation, adhesion, and inflammation in vivo. In vitro cell mesh culture experiments were used to detect cytocompatibility and search for affected biochemical pathways. The PLA-SF mesh was successfully synthesized and did not swell or degrade over time in vitro. It had a high hydrophilicity and strength. The PLA-SF mesh significantly reduced abdominal inflammation and inhibited adhesion formation in rat models. The in vitro degradation rate of the PLA-SF mesh was slower than that of tissue remodeling. Coculture experiments suggested that the PLA-SF mesh reduced the expression of inflammatory factors secreted by fibroblasts and promoted fibroblast proliferation through the TGF-β1/Smad pathway. The PLA-SF mesh had excellent physicochemical properties and biocompatibility, promoted hernia repair of the rat abdominal wall, and reduced postoperative inflammation and adhesion. It is a promising mesh and has potential for clinical application.
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影响因子：
4.6
作者：
Douglass M;Hopkins S;Pandey R;Singha P;Norman M;Handa H
通讯作者：
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影响因子：
4.2
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15.7
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Salaün F
影响因子：
7.8
作者：
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通讯作者：
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