Dentin Regeneration Using Deciduous Pulp Stem/Progenitor Cells
Dentin Regeneration Using Deciduous Pulp Stem/Progenitor Cells
复制标题
使用乳牙髓干/祖细胞进行牙本质再生
DOI：
10.1177/0022034512449834
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发表时间：
2012-06
影响因子：
7.6
通讯作者：
郑颖
中科院分区：
文献类型：
--
作者：
郑颖
修复性牙本质形成对于在疾病或创伤期间维持牙本质结构的完整性至关重要。在这项研究中，我们研究了基于干细胞/祖细胞的组织工程在大型动物模型中的牙本质再生。将猪乳牙髓干/祖细胞（PDPSCs）与β-磷酸三钙（β-TCP）支架混合用于牙本质再生。通过对不同浓度的PDPSCs进行实验，确定牙本质再生的最佳密度。1 mL中5×105 PDPSCs的等量可使附着在支架上的细胞数量最多，碱性磷酸酶活性最高。我们用绿色荧光蛋白（GFP）标记PDPSCs，并将最佳细胞数与β-TCP混合用于修复猪前磨牙髓室顶缺陷。移植后4周，在pdpsc包埋的支架结构中观察到gfp阳性的PDPSCs。移植后16周，PDPSCs与β-TCP混合后，牙本质样结构明显再生，牙髓室顶部缺损几乎完全修复。该研究表明，PDPSC/支架结构可用于直接覆盖牙髓，并为基于干细胞/祖细胞的牙本质再生提供临床前证据。
Reparative dentin formation is essential for maintaining the integrity of dentin structure during disease or trauma. In this study, we investigated stem/progenitor cell-based tissue engineering for dentin regeneration in a large animal model. Porcine deciduous pulp stem/progenitor cells (PDPSCs) were mixed with a beta-tricalcium phosphate (β-TCP) scaffold for dentin regeneration. Different concentrations of PDPSCs were tested to determine the optimal density for dentin regeneration. Aliquots of 5×105 PDPSCs in 1 mL resulted in the highest number of cells attached to the scaffold and the greatest alkaline phosphatase activity. We labeled PDPSCs with green fluorescent protein (GFP) and used the optimal cell numbers mixed with β-TCP to repair pulp chamber roof defects in the premolars of swine. Four weeks after transplantation, GFP-positive PDPSCs were observed in PDPSC-embedded scaffold constructs. At 16 weeks after transplantation, the PDPSCs mixed with β-TCP significantly regenerated the dentin-like structures and nearly completely restored the pulp chamber roof defects. This study demonstrated that the PDPSC/scaffold construct was useful in direct pulp-capping and provides pre-clinical evidence for stem/progenitor cell-based dentin regeneration.
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影响因子：
1.4
作者：
P. Murray;Abeer A Hafez;Anthony J. Smith;C. Cox
通讯作者：
P. Murray;Abeer A Hafez;Anthony J. Smith;C. Cox
影响因子：
7.6
作者：
T. Yamamura
通讯作者：
T. Yamamura
影响因子：
3.8
作者：
Seo BM;Sonoyama W;Yamaza T;Coppe C;Kikuiri T;Akiyama K;Lee JS;Shi S
通讯作者：
Shi S
影响因子：
6.2
作者：
Shi, S;Gronthos, S
通讯作者：
Gronthos, S
影响因子：
5.2
作者：
Brazelton, TR;Blau, HM
通讯作者：
Blau, HM