The number and microlocalization of tumor-associated immune cells are associated with patient's survival time in non-small cell lung cancer.
The number and microlocalization of tumor-associated immune cells are associated with patient's survival time in non-small cell lung cancer.
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DOI：
10.1186/1471-2407-10-220
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发表时间：
2010-05-20
期刊：
影响因子：
3.8
通讯作者：
You Z
中科院分区：
文献类型：
--
作者：
Dai F;Liu L;Che G;Yu N;Pu Q;Zhang S;Ma J;Ma L;You Z
肿瘤微环境由肿瘤细胞、成纤维细胞、内皮细胞和浸润的免疫细胞组成。肿瘤相关免疫细胞可以抑制或促进肿瘤生长和进展。本研究旨在确定非小细胞肺癌中巨噬细胞、成熟树突状细胞和细胞毒性T细胞的数量和微定位是否与患者的生存时间相关。本回顾性研究纳入了99例非小细胞肺癌（NSCLC）患者。使用石蜡包埋的NSCLC标本及其临床病理学数据，包括长达8年的随访信息。进行CD68（巨噬细胞标志物）、CD83（成熟树突状细胞标志物）和CD8（细胞毒性T细胞标志物）的免疫组织化学染色，并以盲法进行评价。在显微镜下计数肿瘤胰岛和间质、肿瘤胰岛或肿瘤间质中的免疫细胞数量。使用社会科学统计软件包（13.0版）分析细胞数量与患者生存时间的相关性。肿瘤间质中巨噬细胞、成熟树突状细胞和细胞毒性T细胞的数量明显多于肿瘤胰岛。在单变量和多变量分析中，肿瘤胰岛中巨噬细胞的数量与患者的生存时间呈正相关，而肿瘤间质中巨噬细胞的数量与患者的生存时间呈负相关。在单变量分析中，肿瘤胰岛和间质、仅肿瘤胰岛或仅肿瘤间质中的成熟树突状细胞的数量与患者的生存时间呈正相关，但在多变量分析中不相关。在单因素分析中，肿瘤胰岛和间质中细胞毒性T细胞的数量与患者的生存时间呈正相关，但在多因素分析中则无相关性。仅肿瘤胰岛或仅间质中的细胞毒性T细胞的数量与患者的生存时间无关。肿瘤胰岛或间质中的巨噬细胞数量是非小细胞肺癌患者生存时间的独立预测因素。计数肿瘤胰岛或间质中的巨噬细胞比计数成熟树突状细胞或细胞毒性T细胞更有助于预测患者的生存时间。
Tumor microenvironment is composed of tumor cells, fibroblasts, endothelial cells, and infiltrating immune cells. Tumor-associated immune cells may inhibit or promote tumor growth and progression. This study was conducted to determine whether the number and microlocalization of macrophages, mature dendritic cells and cytotoxic T cells in non-small cell lung cancer are associated with patient's survival time. Ninety-nine patients with non-small cell lung cancer (NSCLC) were included in this retrospective study. Paraffin-embedded NSCLC specimens and their clinicopathological data including up to 8-year follow-up information were used. Immunohistochemical staining for CD68 (marker for macrophages), CD83 (marker for mature dendritic cells), and CD8 (marker for cytotoxic T cells) was performed and evaluated in a blinded fashion. The numbers of immune cells in tumor islets and stroma, tumor islets, or tumor stroma were counted under a microscope. Correlation of the cell numbers and patient's survival time was analyzed using the Statistical Package for the Social Sciences (version 13.0). The numbers of macrophages, mature dendritic cells and cytotoxic T cells were significantly more in the tumor stroma than in the tumor islets. The number of macrophages in the tumor islets was positively associated with patient's survival time, whereas the number of macrophages in the tumor stroma was negatively associated with patient's survival time in both univariate and multivariate analyses. The number of mature dendritic cells in the tumor islets and stroma, tumor islets only, or tumor stroma only was positively associated with patient's survival time in a univariate analysis but not in a multivariate analysis. The number of cytotoxic T cells in the tumor islets and stroma was positively associated with patient's survival time in a univariate analysis but not in a multivariate analysis. The number of cytotoxic T cells in the tumor islets only or stroma only was not associated with patient's survival time. The number of macrophages in the tumor islets or stroma is an independent predictor of survival time in NSCLC patients. Counting macrophages in the tumor islets or stroma is more useful in predicting patient's survival time than counting mature dendritic cells or cytotoxic T cells.
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影响因子：
8.8
作者：
通讯作者：
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影响因子：
3.8
作者：
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15.3
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影响因子：
45.3
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Bradding, P
影响因子：
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Yang, PC