Arabidopsis acyl-CoA-binding protein ACBP3 participates in plant response to hypoxia by modulating very-long-chain fatty acid metabolism.
Arabidopsis acyl-CoA-binding protein ACBP3 participates in plant response to hypoxia by modulating very-long-chain fatty acid metabolism.
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拟南芥酰基辅酶A结合蛋白ACBP3通过调节极长链脂肪酸代谢参与植物对缺氧的反应
DOI：
10.1111/tpj.12692
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发表时间：
2015-01
期刊：
影响因子：
--
通讯作者：
Xiao S
中科院分区：
文献类型：
--
作者：
Xie LJ;Yu LJ;Chen QF;Wang FZ;Huang L;Xia FN;Zhu TR;Wu JX;Yin J;Liao B;Yao N;Shu W;Xiao S
在拟南芥中，酰基辅酶A结合蛋白(ACBPS)由6个基因(ACBP1到ACBP6)编码，对细胞的各种活动是必不可少的。最近的研究表明，在常氧条件下，膜锚定的ACBPS通过隔离VII族乙烯反应因子参与氧感知。在这里，我们展示了拟南芥ACBP3在低氧耐受中的作用。在黑暗(DS)和光照(LS)条件下，淹水均能显著诱导ACBP3的转录。ACBP3过表达基因(ACBP3-OES)表现出对DS、LS和乙醇胁迫的高敏感性，低氧反应基因的转录减少，过氧化氢在玫瑰花丛中积累。相反，在ACBP3-KOS中抑制ACBP3提高了植物对DS、LS和乙醇处理的耐受性。通过分析OE-1与npr1-5、coi1-2、ein3-1以及CTR1-1突变体的双重组合，我们观察到ACBP3-OES的低氧耐受性减弱依赖于NPR1和CTR1介导的信号通路。脂谱分析显示，在LS暴露下，ACBP3-OES中磷脂酰丝氨酸(C42：2-和C42：3-PS)和葡萄糖肌醇磷酰神经酰胺(C22：0-、C22：1-、C24：0-、C24：1-和C26：1-GIPC)的总量和超长链物种均显著减少，而ACBP3-KOS在LS暴露后显著增加。通过微量热电泳法分析，重组ACBP3蛋白在体外与VLC酰基辅酶A酯结合，亲和力很高。此外，超长链脂肪酸(VLCFA)生物合成的主要调节者MYB30的一个敲除突变体对LS和乙醇胁迫表现出更高的敏感性，这些表型可以通过ACBP3-RNAi得到改善。综上所述，这些发现表明拟南芥ACBP3通过调节VLCFA代谢参与植物对低氧的响应。
In Arabidopsis thaliana, acyl-CoA-binding proteins (ACBPs) are encoded by a family of six genes (ACBP1 to ACBP6), and are essential for diverse cellular activities. Recent investigations suggest that the membrane-anchored ACBPs are involved in oxygen sensing by sequestration of group VII ethylene-responsive factors under normoxia. Here, we demonstrate the involvement of Arabidopsis ACBP3 in hypoxic tolerance. ACBP3 transcription was remarkably induced following submergence under both dark (DS) and light (LS) conditions. ACBP3-overexpressors (ACBP3-OEs) showed hypersensitivity to DS, LS and ethanolic stresses, with reduced transcription of hypoxia-responsive genes as well as accumulation of hydrogen peroxide in the rosettes. In contrast, suppression of ACBP3 in ACBP3-KOs enhanced plant tolerance to DS, LS and ethanol treatments. By analyses of double combinations of OE-1 with npr1-5, coi1-2, ein3-1 as well as ctr1-1 mutants, we observed that the attenuated hypoxic tolerance in ACBP3-OEs was dependent on NPR1- and CTR1-mediated signaling pathways. Lipid profiling revealed that both the total amounts and very-long-chain species of phosphatidylserine (C42:2- and C42:3-PS) and glucosylinositolphosphorylceramides (C22:0-, C22:1-, C24:0-, C24:1-, and C26:1-GIPC) were significantly lower in ACBP3-OEs but increased in ACBP3-KOs upon LS exposure. By microscale thermophoresis analysis, the recombinant ACBP3 protein bound VLC acyl-CoA esters with high affinities in vitro. Further, a knockout mutant of MYB30, a master regulator of very-long-chain fatty acid (VLCFA) biosynthesis, exhibited enhanced sensitivities to LS and ethanolic stresses, phenotypes that were ameliorated by ACBP3-RNAi. Taken together, these findings suggest that Arabidopsis ACBP3 participates in plant response to hypoxia by modulating VLCFA metabolism.
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影响因子：
4.1
作者：
Burton, M;Rose, TM;Knudsen, J
通讯作者：
Knudsen, J
影响因子：
7.2
作者：
Gao, Wei;Li, Hong-Ye;Chye, Mee-Len
通讯作者：
Chye, Mee-Len
DOI：
10.1199/tab.0133
发表时间：
2010-01-01
期刊：
The arabidopsis book
影响因子：
--
作者：
Li-Beisson, Yonghua;Shorrosh, Basil;Ohlrogge, John
通讯作者：
Ohlrogge, John
影响因子：
3.8
作者：
Devaiah, Shivakumar Pattada;Roth, Mary R.;Wang, Xuemin
通讯作者：
Wang, Xuemin
影响因子：
11.6
作者：
Klok, EJ;Wilson, IW;Dennis, ES
通讯作者：
Dennis, ES