Elimination of the Sugar Transporter GAT1 Increased Xylanase I Production in Trichoderma reesei.
Elimination of the Sugar Transporter GAT1 Increased Xylanase I Production in Trichoderma reesei.
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DOI：
10.3389/fmicb.2022.810066
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发表时间：
2022
影响因子：
5.2
通讯作者：
Liu W
中科院分区：
文献类型：
--
作者：
Xu W;Fang Y;Ding M;Ren Y;Meng X;Chen G;Zhang W;Liu W
丝状真菌里氏木霉分泌大量的纤维素酶和半纤维素酶，在工业上有广泛的应用。与对纤维素酶基因表达调控机制的广泛研究相比，对木聚糖酶基因表达调控机制的了解较少。本研究鉴定了几个糖转运蛋白编码基因，这些基因对木聚糖表达显著上调。其中一个基因gat1的缺失导致木聚糖酶产量增加，但几乎不影响纤维素酶的诱导。进一步的分析表明，gat1的缺失在转录水平上显著增加了XYNI的产生，而对XYNII的合成仅产生轻微的影响。相反，过表达gat1导致xyn1表达持续下降。以半乳糖醛酸为唯一碳源培养时，gat1的缺失也影响了xyn1和果胶酶基因的表达。Δgat1及其亲本菌株的转录组分析鉴定出255个差异表达基因，这些基因在糖苷水解酶、脂质代谢、转运蛋白和转录因子类别中富集。结果提示糖转运体GAT1在xyn1表达中具有抑制作用，并揭示了不同木聚糖酶基因的表达可能存在不同的调控机制。
The filamentous fungus Trichoderma reesei secretes large quantities of cellulases and hemicellulases that have found wide applications in industry. Compared with extensive studies on the mechanism controlling cellulase gene expression, less is known about the regulatory mechanism behind xylanase gene expression. Herein, several putative sugar transporter encoding genes that showed significant upregulation on xylan were identified in T. reesei. Deletion of one such gene, gat1, resulted in increased xylanase production but hardly affected cellulase induction. Further analyses demonstrated that deletion of gat1 markedly increased XYNI production at the transcriptional level and only exerted a minor effect on XYNII synthesis. In contrast, overexpressing gat1 caused a continuous decrease in xyn1 expression. Deletion of gat1 also affected the expression of xyn1 and pectinase genes when T. reesei was cultivated with galacturonic acid as the sole carbon source. Transcriptome analyses of Δgat1 and its parental strain identified 255 differentially expressed genes that are enriched in categories of glycoside hydrolases, lipid metabolism, transporters, and transcriptional factors. The results thus implicate a repressive role of the sugar transporter GAT1 in xyn1 expression and reveal that distinct regulatory mechanisms may exist in controlling the expression of different xylanase genes in T. reesei.
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影响因子：
3.7
作者：
Ivanova C;Bååth JA;Seiboth B;Kubicek CP
通讯作者：
Kubicek CP
影响因子：
4.1
作者：
Herrmann, MC;Vrsanska, M;Kubicek, CP
通讯作者：
Kubicek, CP
一种新型转录因子特异性调节里氏木霉中的 GH11 木聚糖酶基因
影响因子：
6.3
作者：
Liu R;Chen L;Jiang Y;Zou G;Zhou Z
通讯作者：
Zhou Z
影响因子：
6.3
作者：
Häkkinen M;Valkonen MJ;Westerholm-Parvinen A;Aro N;Arvas M;Vitikainen M;Penttilä M;Saloheimo M;Pakula TM
通讯作者：
Pakula TM
铜响应启动子的表征及其介导的木聚糖酶调节剂 1 的过表达导致里氏木霉中不依赖于诱导的纤维素酶产生
影响因子：
6.3
作者：
Lv, Xinxing;Zheng, Fanglin;Liu, Weifeng
通讯作者：
Liu, Weifeng