A novel transcription factor specifically regulates GH11 xylanase genes in Trichoderma reesei.
A novel transcription factor specifically regulates GH11 xylanase genes in Trichoderma reesei.
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一种新型转录因子特异性调节里氏木霉中的 GH11 木聚糖酶基因
DOI：
10.1186/s13068-017-0878-x
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发表时间：
2017
影响因子：
6.3
通讯作者：
Zhou Z
中科院分区：
文献类型：
--
作者：
Liu R;Chen L;Jiang Y;Zou G;Zhou Z
里氏木霉(Trichoderma Reesei)是一种广泛用于生产纤维素酶的丝状真菌，但必须提高其木聚糖酶活性以提高木质纤维素对纤维素酶的可及性。几种转录因子在这一过程中发挥了重要作用；然而，几乎所有已报道的转录因子通常同时针对纤维素酶和半纤维素酶基因。结果发现了一个新的锌双核簇转录因子(JGI|Trire2|123881)，它能抑制木聚糖酶活性，但不抑制纤维素酶活性，命名为SxlR(专业木聚糖酶调节因子)。实时定量聚合酶链式反应和凝胶迁移率改变分析表明，SxlR可能与GH11木聚糖酶基因(xyn1、xyn2和xyn5)的启动子结合，而不与GH10(Xyn3)和GH30(Xyn4)木聚糖酶基因的启动子结合，从而直接调控它们的转录和表达。我们还鉴定了SxlR的结合共识序列为5‘-CATCSGSWCWMSA-3’。在里氏木霉RUT-C30中缺失SxlR，产生突变株∆sxlr，使木聚糖酶活性和水解率提高。结论本研究发现了一种新的木聚糖酶基因特异性转录抑制因子，为进一步了解里氏木霉纤维素酶和半纤维素酶的合成和分泌调控系统奠定了基础。SxlR的缺失还可能通过提高木聚糖酶与纤维素酶的比例来提高里氏木霉对木质纤维素的降解效率。
BackgroundThe filamentous fungus Trichoderma reesei is widely utilized in industry for cellulase production, but its xylanase activity must be improved to enhance the accessibility of lignocellulose to cellulases. Several transcription factors play important roles in this progress; however, nearly all the reported transcription factors typically target both cellulase and hemi-cellulase genes. Specific xylanase transcription factor would be useful to regulate xylanase activity directly.ResultsIn this study, a novel zinc binuclear cluster transcription factor (jgi|Trire2|123881) was found to repress xylanase activity, but not cellulase activity, and was designated as SxlR (specialized xylanase regulator). Further investigations using real-time PCR and an electrophoretic mobility shift assay demonstrated that SxlR might bind the promoters of GH11 xylanase genes (xyn1, xyn2, and xyn5), but not those of GH10 (xyn3) and GH30 (xyn4) xylanase genes, and thus regulate their transcription and expression directly. We also identified the binding consensus sequence of SxlR as 5′- CATCSGSWCWMSA-3′. The deletion of SxlR in T. reesei RUT-C30 to generate the mutant ∆sxlr strain resulted in higher xylanase activity as well as higher hydrolytic efficiency on pretreated rice straw.ConclusionsOur study characterizes a novel specific transcriptional repressor of GH11 xylanase genes, which adds to our understanding of the regulatory system for the synthesis and secretion of cellulase and hemi-cellulase in T. reesei. The deletion of SxlR may also help to improve the hydrolytic efficiency of T. reesei for lignocellulose degradation by increasing the xylanase-to-cellulase ratio.
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