Targeted for destruction: degradation of singlet oxygen-damaged chloroplasts.
Targeted for destruction: degradation of singlet oxygen-damaged chloroplasts.
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DOI：
10.1080/15592324.2022.2084955
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发表时间：
2022-12-31
影响因子：
2.9
通讯作者：
Woodson, Jesse D.
中科院分区：
文献类型：
--
作者：
Lemke, Matthew D.;Woodson, Jesse D.
光合作用是植物在动态环境中生存必须调节的重要过程。因此，叶绿体（植物和藻类细胞中进行光合作用的场所）使用多种信号机制向细胞报告其健康状况。人们对此类信号知之甚少，但通常涉及光合作用光反应产生的活性氧（ROS）。一种 ROS，即单线态氧 (1O2)，可以发出信号启动叶绿体降解，但参与识别和降解受损叶绿体的细胞机制（即叶绿体质量控制途径）尚不清楚。为了深入了解这些途径，最近的两项研究调查了过量产生质体亚铁螯合酶二 (fc2) 突变体的拟南芥 1O2 中叶绿体的降解情况。首先，用电子显微镜对降解的叶绿体进行了结构分析，结果表明受损的叶绿体可以突出到中央液泡室中，其结构让人想起裂变型微自噬。 1O2 胁迫的叶绿体在这些相互作用之前膨胀，这可能是它们选择性降解的机制。其次，自噬体和典型自噬（巨自噬）的作用对于 1O2 引发的叶绿体降解来说是可有可无的。相反，假定的裂变型微自噬基因是由叶绿体 1O2 诱导的。在这里，我们讨论这些研究如何暗示这种人们知之甚少的细胞降解途径与 1O2 损坏的叶绿体的分解有关。
Photosynthesis is an essential process that plants must regulate to survive in dynamic environments. Thus, chloroplasts (the sites of photosynthesis in plant and algae cells) use multiple signaling mechanisms to report their health to the cell. Such signals are poorly understood but often involve reactive oxygen species (ROS) produced from the photosynthetic light reactions. One ROS, singlet oxygen (1O2), can signal to initiate chloroplast degradation, but the cellular machinery involved in identifying and degrading damaged chloroplasts (i.e., chloroplast quality control pathways) is unknown. To provide mechanistic insight into these pathways, two recent studies have investigated degrading chloroplasts in the Arabidopsis thaliana 1O2 over-producing plastid ferrochelatase two (fc2) mutant. First, a structural analysis of degrading chloroplasts was performed with electron microscopy, which demonstrated that damaged chloroplasts can protrude into the central vacuole compartment with structures reminiscent of fission-type microautophagy. 1O2-stressed chloroplasts swelled before these interactions, which may be a mechanism for their selective degradation. Second, the roles of autophagosomes and canonical autophagy (macroautophagy) were shown to be dispensable for 1O2-initiated chloroplast degradation. Instead, putative fission-type microautophagy genes were induced by chloroplast 1O2. Here, we discuss how these studies implicate this poorly understood cellular degradation pathway in the dismantling of 1O2-damaged chloroplasts.
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影响因子：
3.5
作者：
Jeran N;Rotasperti L;Frabetti G;Calabritto A;Pesaresi P;Tadini L
通讯作者：
Tadini L
影响因子：
5.6
作者：
Vestergaard CL;Flyvbjerg H;Møller IM
通讯作者：
Møller IM
DOI：
10.1126/science.aac7444
发表时间：
2015-10-23
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Woodson JD;Joens MS;Sinson AB;Gilkerson J;Salomé PA;Weigel D;Fitzpatrick JA;Chory J
通讯作者：
Chory J
影响因子：
4.9
作者：
Fisher, Karen E.;Krishnamoorthy, Praveen;Woodson, Jesse D.
通讯作者：
Woodson, Jesse D.
影响因子：
3.4
作者：
Bienert, Gerd P.;Schjoerring, Jan K.;Jahn, Thomas P.
通讯作者：
Jahn, Thomas P.