Effect of Genistein on vasculogenic mimicry formation by human uveal melanoma cells.
Effect of Genistein on vasculogenic mimicry formation by human uveal melanoma cells.
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金雀异黄素对人葡萄膜黑色素瘤细胞血管生成拟态形成的影响
DOI：
10.1186/1756-9966-28-124
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发表时间：
2009-09-07
影响因子：
11.3
通讯作者：
Wang, Bin
中科院分区：
文献类型：
--
作者：
Cong, Rihong;Sun, Qingmin;Yang, Li;Gu, Haijuan;Zeng, Ying;Wang, Bin
血管生成拟态（vasculogenicmimicry，VM）是一种获得血液供应的侵袭性黑色素瘤。染料木黄酮作为一种潜在的抗癌药物引起了人们的广泛关注。因此，我们研究了金雀异黄素对人葡萄膜黑色素瘤细胞VM的影响。应用PAS染色法检测染料木黄酮作用于三维I型胶原凝胶上培养的葡萄膜黑色素瘤C918细胞后VM结构的变化。采用逆转录聚合酶链反应（RT-PCR）和Western Blot方法检测Genistein对血管内皮钙粘蛋白（VE-cadherin）mRNA和蛋白表达的影响。建立人葡萄膜黑色素瘤C918细胞裸鼠移植瘤模型，采用免疫组化和PAS双染法检测VM的数量。Genistein在体外抑制C918细胞的存活。异位模型研究表明，染料木素在体内可显著降低肿瘤组织切片中的VM。在体外，在对照组、25和50 μM染料木黄酮处理组中发现VM结构，但在100和200 μM处理组中未发现VM结构。RT-PCR和Western Blot结果显示，与对照组相比，100和200 μM Genistein可显著降低C918细胞VE-钙粘蛋白mRNA和蛋白的表达（P < 0.05）。而25和50 μM Genistein则使VE-钙粘蛋白的水平略有降低（P > 0.05）。染料木黄酮抑制葡萄膜黑色素瘤细胞VM形成的体内和体外研究。Genistein抑制葡萄膜黑色素瘤VM形成的一个可能的潜在分子机制与VE-钙粘蛋白的下调有关。
Vasculogenic mimicry (VM) was increasingly recognized as a form of aggressive melanoma acquiring blood supply. Genistein had attracted much attention as a potential anticancer agent. Therefore, we examined the effect of Genistein on VM in human uveal melanoma cells. VM structure was detected by periodic acid-Schiff (PAS) staining for uveal melanoma C918 cells cultured on the three-dimensional type I collagen gels after exposed to Genistein. We used reverse transcription polymerase chain reaction (RT-PCR) and Western Blot analysis to examine the effect of Genistein on vascular endothelial cadherin (VE-cadherin) mRNA and protein expression. The nude mice models of human uveal melanoma C918 cells were established to assess the number of VM using immunohistochemical and PAS double-staining. Genistein inhibited the survival of C918 cells in vitro. The ectopic model study showed that VM in tumor tissue sections were significantly reduced by Genistein in vivo. In vitro, the VM structure was found in control, 25 and 50 μM Genistein-treatment groups but not in 100 and 200 μM. RT-PCR and Western Blot showed that 100 and 200 μM concentration of Genistein could significantly decrease VE-cadherin mRNA and protein expression of C918 cells compared with control (P < 0.05). However, the 25 and 50 μM Genistein slightly decreased the VE-cadherin level in vitro (P > 0.05). Genistein inhibits VM formation of uveal melanoma cells in vivo and in vitro. One possible underlying molecular mechanism by which Genistein could inhibit VM formation of uveal melanoma is related to down-regulation of VE-cadherin.
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影响因子：
2.9
作者：
Lian, FR;Bhuiyan, M;Sarkar, FH
通讯作者：
Sarkar, FH
影响因子：
5.6
作者：
Pepper, MS;Hazel, SJ;Schleuning, WD
通讯作者：
Schleuning, WD
影响因子：
6.4
作者：
Shirakawa, K;Wakasugi, H;Konishi, F
通讯作者：
Konishi, F
影响因子：
3.8
作者：
Dixon, RA;Ferreira, D
通讯作者：
Ferreira, D
DOI：
10.1080/10623320600903940
发表时间：
2006-07-01
影响因子：
--
作者：
Piao, Meihua;Mori, Daisuke;Tokunaga, Osamu
通讯作者：
Tokunaga, Osamu