Normal expression of insect-resistant transgene in progeny of common wild rice crossed with genetically modified rice: its implication in ecological biosafety assessment
Normal expression of insect-resistant transgene in progeny of common wild rice crossed with genetically modified rice: its implication in ecological biosafety assessment
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普通野生稻与转基因水稻杂交后代抗虫转基因的正常表达及其对生态生物安全评估的意义
DOI：
10.1007/s00122-009-1075-5
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发表时间：
2009-06
影响因子：
5.4
通讯作者：
Wang, Feng
中科院分区：
文献类型：
--
作者：
Chen, Rui;Lu, Bao-Rong;Xia, Hui;Su, Jun;Rong, Jun;Song, Zhiping;Wang, Feng
转基因水稻向其野生近缘种的基因外溢可能造成不良的生态后果。了解基因流动后野生稻中的转基因表达水平对于评估这些后果非常重要，因为转基因水稻的转基因逃逸是无法阻止的。为了确定一个转基因基因在普通野生稻（Oryza rufipogon）中的表达，我们采用三明治酶联免疫吸附法分析了3个含有aBttransgene的转基因水稻品系、它们与普通野生稻的f1杂交品系和f2后代在不同生育期的Cry1Ac蛋白含量。在整个生育期，野生水稻叶片样品Cry1Ac蛋白的平均含量在0.016 ~ 0.069%之间，茎中Cry1Ac蛋白的平均含量在0.12 ~ 0.39%之间。f1杂交和f2子代个体间Cry1Ac蛋白含量差异较大，部分野生个体的bttoxin含量高于栽培型转基因水稻。结果表明，该基因在抗虫转基因稻与普通野生稻的种间杂交种中可以正常表达，对目标昆虫的影响可能与在转基因稻中相似。
Transgene outflow from genetically modified (GM) rice to its wild relatives may cause undesirable ecological consequences. Understanding the level of transgene expression in wild rice following gene flow is important for assessing such consequences, providing that transgene escape from GM rice cannot be prevented. To determine the expression of a transgene in common wild rice (Oryza rufipogon), we analyzed the content of Cry1Ac protein in three GM rice lines containing aBttransgene, their F1hybrids with common wild rice and F2progeny at different growth stages, using the sandwich enzyme-linked immunosorbent assay. The average content of Cry1Ac protein in leaf samples of the wild rice lines ranged between 0.016 and 0.069% during the entire growth period, whereas that in stems varied between 0.12 and 0.39%. A great variation in Cry1Ac protein content was detected among individuals of F1hybrids and F2progeny, with some wild individuals showing higher level ofBttoxin than the cultivated GM rice. The results suggest that theBttransgene can express normally in the interspecific hybrids between insect-resistant GM rice and common wild rice, and may have similar effects on the target insects as in GM rice.
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影响因子：
2.2
作者：
Tabashnik, BE;Carrière, Y;Zhao, JZ
通讯作者：
Zhao, JZ
影响因子：
3.1
作者：
Yan Sheng Liu;J. S. Levin;Jeffrey S. Murray;E. A. Wernsman;A. Weissinger
通讯作者：
Yan Sheng Liu;J. S. Levin;Jeffrey S. Murray;E. A. Wernsman;A. Weissinger
影响因子：
13.8
作者：
Feng Wang;Q. Yuan;Lei Shi;Q. Qian;Wuge Liu;Ba-Geng Kuang;D. Zeng;Y. Liao;B. Cao;S. Jia
通讯作者：
Feng Wang;Q. Yuan;Lei Shi;Q. Qian;Wuge Liu;Ba-Geng Kuang;D. Zeng;Y. Liao;B. Cao;S. Jia
影响因子：
5.4
作者：
Bao-Rong Lu;K. Zheng;H. Qian;J. Zhuang
通讯作者：
Bao-Rong Lu;K. Zheng;H. Qian;J. Zhuang
影响因子：
5.2
作者：
Mohammad Firoz Alam;K. Datta;E. Abrigo;A. Vasquez;D. Senadhira;S. Datta
通讯作者：
Mohammad Firoz Alam;K. Datta;E. Abrigo;A. Vasquez;D. Senadhira;S. Datta