Escherichia coli low-molecular-weight penicillin-binding proteins help orient septal FtsZ, and their absence leads to asymmetric cell division and branching.
Escherichia coli low-molecular-weight penicillin-binding proteins help orient septal FtsZ, and their absence leads to asymmetric cell division and branching.
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DOI：
10.1111/j.1365-2958.2012.08023.x
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发表时间：
2012-04
影响因子：
3.6
通讯作者：
Young KD
中科院分区：
文献类型：
--
作者：
Potluri LP;de Pedro MA;Young KD
缺乏低分子青霉素结合蛋白(LMW PBPs)的大肠杆菌细胞表现出形态变化，这种变化也出现在隔膜蛋白FtsZ错误定位的情况下，这表明肽聚糖修饰和分裂可能共同作用产生细胞形状。我们发现，在缺乏PBP5和其他LMW PBP的菌株中，较高的FtsZ浓度使分支细胞和错误定向的Z环的频率增加了10-15倍。这些环的内陷产生了方向不正确的隔膜，这反过来又产生了不对称的细胞极点，最终伸长成枝条。分支总是起源于异常间隔事件的残留物，巩固了异常细胞分裂与分支形成之间的关系。在没有PBP5的情况下，PBP6和DacD定位于新生的隔膜，提示这些PBPs可以部分替代PBP5的丢失。我们认为，当错误定位的FtsZ触发在细胞分裂过程中在不寻常的位置插入惰性肽聚糖时，分支就开始了。只有在正常的细胞壁伸长将斑块分开后，才能看到分支。因此，LMW PBPs和细胞质FtsZ之间的关系最终影响细胞分裂和整体形状。
Escherichia coli cells lacking low molecular weight penicillin binding proteins (LMW PBPs) exhibit morphological alterations that also appear when the septal protein FtsZ is mislocalized, suggesting that peptidoglycan modification and division may work together to produce cell shape. We found that in strains lacking PBP5 and other LMW PBPs, higher FtsZ concentrations increased the frequency of branched cells and incorrectly oriented Z rings by 10- to 15-fold. Invagination of these rings produced improperly oriented septa, which in turn gave rise to asymmetric cell poles that eventually elongated into branches. Branches always originated from the remnants of abnormal septation events, cementing the relationship between aberrant cell division and branch formation. In the absence of PBP5, PBP6 and DacD localized to nascent septa, suggesting that these PBPs can partially substitute for the loss of PBP5. We propose that branching begins when mislocalized FtsZ triggers the insertion of inert peptidoglycan at unusual positions during cell division. Only later, after normal cell wall elongation separates the patches, do branches become visible. Thus, a relationship between the LMW PBPs and cytoplasmic FtsZ ultimately affects cell division and overall shape.
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影响因子：
3.2
作者：
de Pedro, MA;Grünfelder, CG;Schwarz, H
通讯作者：
Schwarz, H
影响因子：
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影响因子：
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影响因子：
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Aarsman, MEG;Piette, A;den Blaauwen, T
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