Characterization of a recombinant coronavirus infectious bronchitis virus with distinct S1 subunits of spike and nucleocapsid genes and a 3' untranslated region.
Characterization of a recombinant coronavirus infectious bronchitis virus with distinct S1 subunits of spike and nucleocapsid genes and a 3' untranslated region.
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DOI：
10.1016/j.vetmic.2012.10.027
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发表时间：
2013-03-23
影响因子：
3.3
通讯作者：
Liu S
中科院分区：
文献类型：
--
作者：
Liu X;Ma H;Xu Q;Sun N;Han Z;Sun C;Guo H;Shao Y;Kong X;Liu S
从一只接种 H120 疫苗的鸡中分离出一种传染性支气管炎病毒 (IBV)，ck/CH/LZJ/111113，该鸡表现出疑似 IBV 感染的疾病。中和试验表明ck/CH/LZJ/111113与中国主要IBV LX4型或Mass型疫苗株不同。系统发育分析证实ck/CH/LZJ/111113属于4/91型；然而，对全长基因组的进一步广泛分析发现了重组事件的发生。因此，ck/CH/LZJ/111113起源于ck/CH/LDL/091022-和4/91-样菌株之间的重组事件，分别位于刺突(S)糖蛋白基因上游的三个开关位点以及S1和核衣壳(N)基因的3'端。 ck/CH/LZJ/111113 和 ck/CH/LDL/091022 之间的血清型和肾脏组织向性差异可能是由于来自 4/91 样毒株的 ck/CH/LDL/091022 样病毒摄取 S1 基因所致。这种重组事件发生在 N 基因的 3' 端，3' 非翻译区可能是两种病毒接种的鸡组织中复制效率差异的原因。
An infectious bronchitis virus (IBV), ck/CH/LZJ/111113, was isolated from a H120-vaccinated chicken which showed disease suspected of IBV infection. Neutralization testing showed that ck/CH/LZJ/111113 was distinct from either the Chinese predominant IBV LX4-type or Mass-type vaccine strains. Phylogenetic analysis confirmed that ck/CH/LZJ/111113 is of the 4/91 type; however, further extensive analyses of full-length genomes identified occurrence of recombination events. Therefore, ck/CH/LZJ/111113 originated from the recombination events between ck/CH/LDL/091022- and 4/91-like strains at three switch sites located upstream of the spike (S) glycoprotein gene, and the 3′ ends of S1 and nuceocapsid (N) genes, respectively. The difference of serotypes and tissue tropisms in kidneys between ck/CH/LZJ/111113 and ck/CH/LDL/091022 may have been contributed by the uptake of the S1 gene by a ck/CH/LDL/091022-like virus from a 4/91-like strain. This recombination event took place at the 3′ end of the N gene and the 3′ untranslated region may account for differences in replication efficiency in tissues of chickens inoculated by the two viruses.
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影响因子：
3.7
作者：
Jackwood MW;Boynton TO;Hilt DA;McKinley ET;Kissinger JC;Paterson AH;Robertson J;Lemke C;McCall AW;Williams SM;Jackwood JW;Byrd LA
通讯作者：
Byrd LA
影响因子：
4.3
作者：
Jones, R. M.;Ellis, R. J.;Wakeley, P. R.
通讯作者：
Wakeley, P. R.
影响因子：
--
作者：
Masters PS
通讯作者：
Masters PS
在OVO中与异源免疫血清不完全中和后，流感病毒的持续性抗原变异。
影响因子：
15.3
作者：
ARCHETTI, I;HORSFALL, F L Jr
通讯作者：
HORSFALL, F L Jr
DOI：
10.1074/jbc.m104097200
发表时间：
2001-08-31
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Ziebuhr J;Thiel V;Gorbalenya AE
通讯作者：
Gorbalenya AE