Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples.
Sensitive, smartphone-based SARS-CoV-2 detection from clinical saline gargle samples.
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DOI：
10.1093/pnasnexus/pgac028
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Yoon, Jeong-Yeol
中科院分区：
文献类型：
--
作者：
Breshears, Lane E.;Nguyen, Brandon T.;Akarapipad, Patarajarin;Sosnowski, Katelyn;Kaarj, Kattika;Quirk, Grace;Uhrlaub, Jennifer L.;Nikolich-Zugich, Janko;Worobey, Michael;Yoon, Jeong-Yeol
关键词：
唾液标本由于易于收集和降低医疗保健提供者的风险而引起了对呼吸道病毒感染诊断的兴趣。然而，由于这些标本的黏性和低病毒载量，快速和敏感的免疫测定尚未得到令人满意的证明。利用纸质微流控芯片和基于智能手机的荧光显微镜，我们开发了一种高灵敏度、低成本的临床生理盐水含漱液中SARS-CoV-2免疫荧光微粒测定方法。我们证明了检测限为10 ag/μL。对于易于收集的生理盐水含漱液样本，我们的临床敏感性、特异性和准确性分别为100%、86%和93%，其中n = 27名人类受试者中n = 13名RT-qPCR阳性。
Saliva specimens have drawn interest for diagnosing respiratory viral infections due to their ease of collection and decreased risk to healthcare providers. However, rapid and sensitive immunoassays have not yet been satisfactorily demonstrated for such specimens due to their viscosity and low viral loads. Using paper microfluidic chips and a smartphone-based fluorescence microscope, we developed a highly sensitive, low-cost immunofluorescence particulometric SARS-CoV-2 assay from clinical saline gargle samples. We demonstrated the limit of detection of 10 ag/μL. With easy-to-collect saline gargle samples, our clinical sensitivity, specificity, and accuracy were 100%, 86%, and 93%, respectively, for n = 27 human subjects with n = 13 RT-qPCR positives.
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