Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR.
Weigh Biomaterials by Quantifying Species-specific DNA with Real-time PCR.
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通过实时 PCR 定量物种特异性 DNA 来称重生物材料
DOI：
10.1038/s41598-017-05083-9
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发表时间：
2017-07-06
影响因子：
4.6
通讯作者：
Tang Z
中科院分区：
文献类型：
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作者：
Chen R;Wang J;Yuan Y;Deng Y;Lai X;Du F;Dong J;Huang X;Cui X;Tang Z
标签上的东西不是瓶子里的东西，从食品到草药产品（HMP），经济动机的生物材料掺假是影响食品和药品行业的长期问题。准确识别加工商品中的生物材料成分是非常需要的。在这一领域，基于DNA的技术已被证明是克服定性挑战的强大工具。然而，是否可以用PCR来量化生物材料的重量？因此，需要回答一个基本的科学问题：DNA含量与生物材料质量之间的关系是什么？DNA含量是否像大多数研究人员以前认为的那样与生物材料的质量成正比？在本研究中，我们首次发现，在完全控制分析操作的情况下，生物材料的DNA含量与其重量之间确实存在线性关系。在这种情况下，高度加工的商业产品中靶向生物材料的质量也可以通过经典的实时PCR以良好的再现性定量物种特异性DNA来计算。
What’s on the label is not what’s in the bottle, from food products to herbal medicinal products (HMPs), economically-motivated biomaterials adulteration is a long-term problem affecting the food and drug industry. Accurate identification of the biomaterial ingredients in processed commodities is highly desirable. In this field, DNA-based techniques have proved to be powerful tools to overcome qualitative challenges. However, is it possible to quantify the weight of biological materials with PCR? Therefore, a basic scientific question needs to be answered: what’s the relationship between DNA content and the mass of biological materials? Is DNA content directly proportional to the mass of biological materials as most of the researchers previously thought? In this study, we firstly found that there exists a linear relation between DNA contents and the weight of biomaterials indeed when the analytical practices are fully controlled. In this case, the mass of targeted biomaterials in the highly processed commercial products can also be calculated by quantifying the species-specific DNA through classic real-time PCR with a good reproducibility.
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影响因子：
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作者：
Jiang C;Cao L;Yuan Y;Chen M;Jin Y;Huang L
通讯作者：
Huang L
影响因子：
8.8
作者：
Garino, Cristiano;De Paolis, Angelo;Arlorio, Marco
通讯作者：
Arlorio, Marco
影响因子：
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作者：
Kiebala M;Polesskaya O;Yao Z;Perry SW;Maggirwar SB
通讯作者：
Maggirwar SB
影响因子：
8.8
作者：
Fang, Xin;Zhang, Chi
通讯作者：
Zhang, Chi
影响因子：
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作者：
Newmaster SG;Grguric M;Shanmughanandhan D;Ramalingam S;Ragupathy S
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