Untangling the web: the diverse functions of the PIWI/piRNA pathway.
Untangling the web: the diverse functions of the PIWI/piRNA pathway.
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解开网络：PIWI/piRNA 途径的多种功能。
DOI：
10.1002/mrd.22195
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发表时间：
2013-08
影响因子：
2.5
通讯作者：
Juliano, Celina E.
中科院分区：
文献类型：
--
作者：
Mani, Sneha Ramesh;Juliano, Celina E.
小 RNA 通过基因调控影响多种细胞过程。 Argonaute 蛋白与小 RNA 结合形成效应复合物，控制转录和转录后基因表达。 PIWI 蛋白属于 Argonaute 蛋白家族，并结合 PIWI 相互作用 RNA (piRNA)。它们在种系中高度丰富，但也在一些体细胞组织中表达。 PIWI/piRNA 通路在果蝇的转座子抑制中发挥作用，这种抑制是通过表观遗传调控和转座子 mRNA 的转录后降解而发生的。这些功能是保守的，但物种之间转座子抑制的程度和机制存在明显差异。 piwi 基因突变导致转座子 mRNA 上调。据推测，这种增加的转座子动员导致基因组不稳定，从而导致不育，尽管转座子上调和基因组不稳定之间尚未建立因果关系。另一种情况可能是 piwi 突变直接影响基因组不稳定性，从而导致转座子表达增加。我们提出PIWI/piRNA途径通过多种方式控制基因组稳定性：抑制转座子、直接调节染色质结构以及调节控制与基因组稳定性相关的重要生物过程的基因。 PIWI/piRNA 通路还调节至少一些（如果不是很多的话）蛋白质编码基因，这进一步支持了 piwi 基因可能具有除转座子抑制之外更广泛的功能的观点。一个有趣的可能性是，PIWI/piRNA 途径正在使用转座子序列来协调大组基因的表达，从而调节细胞功能。
Small RNAs impact several cellular processes through gene regulation. Argonaute proteins bind small RNAs to form effector complexes that control transcriptional and post-transcriptional gene expression. PIWI proteins belong to the Argonaute protein family, and bind PIWI-interacting RNAs (piRNAs). They are highly abundant in the germline, but are also expressed in some somatic tissues. The PIWI/piRNA pathway has a role in transposon repression in Drosophila, which occurs both by epigenetic regulation and post-transcriptional degradation of transposon mRNAs. These functions are conserved, but clear differences in the extent and mechanism of transposon repression exist between species. Mutations in piwi genes lead to the upregulation of transposon mRNAs. It is hypothesized that this increased transposon mobilization leads to genomic instability and thus sterility, although no causal link has been established between transposon upregulation and genome instability. An alternative scenario could be that piwi mutations directly affect genomic instability, and thus lead to increased transposon expression. We propose that the PIWI/piRNA pathway controls genome stability in several ways: suppression of transposons, direct regulation of chromatin architecture and regulation of genes that control important biological processes related to genome stability. The PIWI/piRNA pathway also regulates at least some, if not many, protein-coding genes, which further lends support to the idea that piwi genes may have broader functions beyond transposon repression. An intriguing possibility is that the PIWI/piRNA pathway is using transposon sequences to coordinate the expression of large groups of genes to regulate cellular function.
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影响因子：
64.5
作者：
Ashe A;Sapetschnig A;Weick EM;Mitchell J;Bagijn MP;Cording AC;Doebley AL;Goldstein LD;Lehrbach NJ;Le Pen J;Pintacuda G;Sakaguchi A;Sarkies P;Ahmed S;Miska EA
通讯作者：
Miska EA
影响因子：
64.5
作者：
BINGHAM, PM;KIDWELL, MG;RUBIN, GM
通讯作者：
RUBIN, GM
DOI：
10.1007/bf00332411
发表时间：
1989-09-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
BRADSHAW, VA;MCENTEE, K
通讯作者：
MCENTEE, K
影响因子：
30.8
作者：
Bentwich, I;Avniel, A;Bentwich, Z
通讯作者：
Bentwich, Z
影响因子：
2.7
作者：
Alie, Alexandre;Leclere, Lucas;Manuel, Michael
通讯作者：
Manuel, Michael