Plasmids manipulate bacterial behaviour through translational regulatory crosstalk.
Plasmids manipulate bacterial behaviour through translational regulatory crosstalk.
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DOI：
10.1371/journal.pbio.3001988
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发表时间：
2023-02
期刊：
影响因子：
9.8
通讯作者：
Malone, Jacob G.
中科院分区：
文献类型：
--
作者：
Thompson, Catriona M. A.;Hall, James P. J.;Chandra, Govind M.;Martins, Carlo;Saalbach, Gerhard H.;Panturat, Supakan;Bird, Susannah M.;Ford, Samuel W.;Little, Richard H. A.;Piazza, Ainelen G.;Harrison, Ellie;Jackson, Robert W.;Brockhurst, Michael A.;Malone, Jacob G.
除了在水平基因转移中的作用外，接合质粒通常编码细菌调节因子的同源物。已知的质粒调节因子同源物对特定细菌性状的转录具有高度靶向作用。在这里，我们发现了一个质粒翻译调节因子RsmQ，能够在荧光假单胞菌中进行全局调控，并引起从能动到固着生活方式的行为转变。RsmQ作为一个全局调节因子，通过与宿主mRNA的直接相互作用和干扰宿主的翻译调控网络来控制宿主蛋白质组。这种mRNA干扰导致代谢基因、关键调节因子和参与趋化性的基因的大规模蛋白质组变化，从而控制细菌的代谢和运动。此外，比较分析发现RsmQ编码的大量不同的质粒分离的多个细菌宿主类群，这表明广泛的重要性，RsmQ操纵细菌的行为在临床，环境和农业生态位。RsmQ是一种广泛存在的质粒全局翻译调节因子，主要进化用于宿主染色体控制以操纵细菌的行为和生活方式。质粒含有细菌信号蛋白的同源物，并利用它们操纵宿主细胞使其受益。这项研究描述了一种广泛的质粒携带的翻译调节剂，颠覆土壤细菌的生态特性，诱导社区组织和促进质粒传播。
Beyond their role in horizontal gene transfer, conjugative plasmids commonly encode homologues of bacterial regulators. Known plasmid regulator homologues have highly targeted effects upon the transcription of specific bacterial traits. Here, we characterise a plasmid translational regulator, RsmQ, capable of taking global regulatory control in Pseudomonas fluorescens and causing a behavioural switch from motile to sessile lifestyle. RsmQ acts as a global regulator, controlling the host proteome through direct interaction with host mRNAs and interference with the host’s translational regulatory network. This mRNA interference leads to large-scale proteomic changes in metabolic genes, key regulators, and genes involved in chemotaxis, thus controlling bacterial metabolism and motility. Moreover, comparative analyses found RsmQ to be encoded on a large number of divergent plasmids isolated from multiple bacterial host taxa, suggesting the widespread importance of RsmQ for manipulating bacterial behaviour across clinical, environmental, and agricultural niches. RsmQ is a widespread plasmid global translational regulator primarily evolved for host chromosomal control to manipulate bacterial behaviour and lifestyle. Plasmids contain homologs of bacterial signaling proteins and use them to manipulate host cells to their benefit. This study describes a widespread plasmid-borne translational regulator that subverts ecological traits in soil bacteria, inducing community organization and promoting plasmid transmission.
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影响因子：
2.1
作者：
Grenga L;Little RH;Malone JG
通讯作者：
Malone JG
影响因子：
16.6
作者：
Cazares, Adrian;Moore, Matthew P.;Winstanley, Craig
通讯作者：
Winstanley, Craig
DOI：
10.1080/2159256x.2016.1179074
发表时间：
2016-05
期刊：
Mobile genetic elements
影响因子：
--
作者：
Harrison E;Dytham C;Hall JP;Guymer D;Spiers AJ;Paterson S;Brockhurst MA
通讯作者：
Brockhurst MA
影响因子：
14.9
作者：
Galata V;Fehlmann T;Backes C;Keller A
通讯作者：
Keller A
影响因子：
14.9
作者：
Duss O;Michel E;Diarra dit Konté N;Schubert M;Allain FH
通讯作者：
Allain FH