Genetic, morphometric, and molecular analyses of interspecies differences in head shape and hybrid developmental defects in the wasp genus Nasonia.
Genetic, morphometric, and molecular analyses of interspecies differences in head shape and hybrid developmental defects in the wasp genus Nasonia.
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DOI：
10.1093/g3journal/jkab313
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发表时间：
2021-12-08
期刊：
影响因子：
--
通讯作者：
Lynch JA
中科院分区：
文献类型：
--
作者：
Cohen LB;Jewell R;Moody D;Arsala D;Werren JH;Lynch JA
寄生蜂纳索尼亚属的雄性具有独特的、物种特异性的头部形状。物种间可育杂种的可用性，沿着雄性的专性单倍性，有助于分析发育和进化中复杂的基因相互作用。先前的分析表明，无论是在头的形状之间的分歧，丽翅夜蛾和Nasonia giraulti，和F2单倍体杂交雄性的头部特异性发育缺陷，是由相互作用的基因网络的多重变化。在这里，我们扩展我们的理解，影响男性头部形态发生的基因相互作用。人工二倍体雄性杂种的应用表明，介导发育缺陷的等位基因是隐性的，而其他头形性状之间存在不同的显性关系。在分子水平上，性别决定位点doublesex在男性头部形状差异中起主要作用，但它不是唯一的重要因素。第2号染色体上的长颈鹿区域的渐渗揭示了一个隐性基因座，该基因座会导致尖酸刻薄背景下雄性和雌性的完全渗透性头裂。第三个物种（N。longicornis）进行了头形态相关遗传变化的时间研究，发现引起缺陷的大多数变化发生在N. vitripennis与其它种的分化，但先于N. giraulti和N. longicornis从对方。我们的研究结果表明，发育基因网络可以解剖使用种间杂交在纳索尼亚，并为未来的精细规模的头部形状和杂交发育缺陷的遗传解剖奠定了基础。
Males in the parasitoid wasp genus Nasonia have distinct, species-specific, head shapes. The availability of fertile hybrids among the species, along with obligate haploidy of males, facilitates analysis of complex gene interactions in development and evolution. Previous analyses showed that both the divergence in head shape between Nasonia vitripennis and Nasonia giraulti, and the head-specific developmental defects of F2 haploid hybrid males, are governed by multiple changes in networks of interacting genes. Here, we extend our understanding of the gene interactions that affect morphogenesis in male heads. Use of artificial diploid male hybrids shows that alleles mediating developmental defects are recessive, while there are diverse dominance relationships among other head shape traits. At the molecular level, the sex determination locus doublesex plays a major role in male head shape differences, but it is not the only important factor. Introgression of a giraulti region on chromsome 2 reveals a recessive locus that causes completely penetrant head clefting in both males and females in a vitripennis background. Finally, a third species (N. longicornis) was used to investigate the timing of genetic changes related to head morphology, revealing that most changes causing defects arose after the divergence of N. vitripennis from the other species, but prior to the divergence of N. giraulti and N. longicornis from each other. Our results demonstrate that developmental gene networks can be dissected using interspecies crosses in Nasonia, and set the stage for future fine-scale genetic dissection of both head shape and hybrid developmental defects.
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影响因子：
3.8
作者：
Hoedjes, K. M.;Smid, H. M.;Vet, L. E. M.;Werren, J. H.
通讯作者：
Werren, J. H.
影响因子：
3.7
作者：
Beukeboom LW;Koevoets T;Morales HE;Ferber S;van de Zande L
通讯作者：
van de Zande L
影响因子：
3.8
作者：
Desjardins, C. A.;Perfectti, F.;Bartos, J. D.;Enders, L. S.;Werren, J. H.
通讯作者：
Werren, J. H.
DOI：
10.1016/j.cub.2018.04.010
发表时间：
2018-06-04
期刊：
Current biology : CB
影响因子：
--
作者：
Funkhouser-Jones LJ;van Opstal EJ;Sharma A;Bordenstein SR
通讯作者：
Bordenstein SR
影响因子：
3.8
作者：
Aparicio, JM;Bonal, R
通讯作者：
Bonal, R