Semipersistently Transmitted, Phloem Limited Plant Viruses Are Inoculated during the First Subphase of Intracellular Stylet Penetrations in Phloem Cells.
Semipersistently Transmitted, Phloem Limited Plant Viruses Are Inoculated during the First Subphase of Intracellular Stylet Penetrations in Phloem Cells.
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DOI：
10.3390/v13010137
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发表时间：
2021-01-19
期刊：
影响因子：
--
通讯作者：
Fereres A
中科院分区：
文献类型：
--
作者：
Jiménez J;Moreno A;Fereres A
绿色Myzus persicae Sulzer是半持续传播和韧皮部限制的甜菜黄化病毒（BYV，Closterovirus）的主要媒介。监测M.利用电子穿透图（EPG）技术对桃蚜的探测行为进行了研究，结果表明BYV的接种发生在伴随细胞和/或筛元细胞中产生的独特的短暂细胞内穿孔（韧皮部-pds）期间。细胞内针穿刺（或pds）被细分为三个亚期（II-1，II-2和II-3），这与非韧皮部限制的病毒以非持久性或半持久性方式传播的传递或吸收有关。与非韧皮部限制性病毒相反，蚜虫成功传递韧皮部限制性病毒所涉及的特定pd亚相仍然未知。因此，我们对携带BYV的M.用EPG技术对甜菜植株中的桃蚜（Persicae）个体进行了研究，并在每个韧皮部-pd亚期人工终止了取食过程。结果表明，蚜虫，只执行亚阶段II-1的韧皮部-PD传播BYV在类似的效率比那些允许执行亚阶段II-2或完整的韧皮部-PD。这一结果表明，BYV接种发生在韧皮部-pd的第一个亚阶段。讨论了BYV在韧皮部细胞中传递的具体传递机制。
The green peach aphid Myzus persicae Sulzer is the main vector of the semipersistently transmitted and phloem-limited Beet yellows virus (BYV, Closterovirus). Studies monitoring the M. persicae probing behavior by using the Electrical penetration graphs (EPG) technique revealed that inoculation of BYV occurs during unique brief intracellular punctures (phloem-pds) produced in companion and/or sieve element cells. Intracellular stylet punctures (or pds) are subdivided in three subphases (II-1, II-2 and II-3), which have been related to the delivery or uptake of non-phloem limited viruses transmitted in a non-persistent or semipersistent manner. As opposed to non-phloem limited viruses, the specific pd subphase(s) involved in the successful delivery of phloem limited viruses by aphids remain unknown. Therefore, we monitored the feeding process of BYV-carrying M. persicae individuals in sugar beet plants by the EPG technique and the feeding process was artificially terminated at each phloem-pd subphase. Results revealed that aphids that only performed the subphase II-1 of the phloem-pd transmitted BYV at similar efficiency than those allowed to perform subphase II-2 or the complete phloem-pd. This result suggests that BYV inoculation occurs during the first subphase of the phloem-pd. The specific transmission mechanisms involved in BYV delivery in phloem cells are discussed.
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影响因子：
1.9
作者：
SCHELLER, HV;SHUKLE, RH
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