Fast kinase domain-containing protein 3 is a mitochondrial protein essential for cellular respiration.-猫眼课题宝

DOI：

10.1016/j.bbrc.2010.09.075

发表时间：

2010-10-22

期刊：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

影响因子：

3.1

通讯作者：

中科院分区：

生物学4区

文献类型：

作者：

Simarro, Maria;Gimenez-Cassina, Alfredo;Kedersha, Nancy;Lazaro, Jean-Bernard;Adelmant, Guillaume O.;Marto, Jarrod A.;Rhee, Kirsten;Tisdale, Sarah;Danial, Nika;Benarafa, Charaf;Orduna, Anonio;Anderson, Paul

关键词：

FASTKD2 FASTKD3 Mitochondria Respiration Interactome Mitochondrial respiratory chain complexes

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2963690/?report=classic

Fas-activated serine/threonine phosphoprotein (FAST) is the founding member of the FAST kinase domain-containing protein (FASTKD) family that includes FASTKD1-5. FAST is a sensor of mitochondrial stress that modulates protein translation to promote the survival of cells exposed to adverse conditions. Mutations in FASTKD2 have been linked to a mitochondrial encephalomyopathy that is associated with reduced cytochrome c oxidase activity, an essential component of the mitochondrial electron transport chain. We have confirmed the mitochondrial localization of FASTKD2 and shown that all FASTKD family members are found in mitochondria. Although human and mouse FASTKD1-5 genes are expressed ubiquitously, some of them are most abundantly expressed in mitochondria-enriched tissues. We have found that RNA interference-mediated knockdown of FASTKD3 severely blunts basal and stress-induced mitochondrial oxygen consumption without disrupting the assembly of respiratory chain complexes. Tandem affinity purification reveals that FASTKD3 interacts with components of mitochondrial respiratory and translation machineries. Our results introduce FASTKD3 as an essential component of mitochondrial respiration that may modulate energy balance in cells exposed to adverse conditions by functionally coupling mitochondrial protein synthesis to respiration.