Early Integration of Temperature and Humidity Stimuli in the Drosophila Brain.
Early Integration of Temperature and Humidity Stimuli in the Drosophila Brain.
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DOI：
10.1016/j.cub.2017.06.077
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发表时间：
2017-08-07
期刊：
影响因子：
--
通讯作者：
Gallio M
中科院分区：
文献类型：
--
作者：
Frank DD;Enjin A;Jouandet GC;Zaharieva EE;Para A;Stensmyr MC;Gallio M
果蝇触角含有机械、嗅觉、热和湿度刺激的受体神经元。表达离子型受体IR 40 a的神经元已经被牵涉到适当湿度范围的选择，但是虽然先前的工作表明昆虫湿感受器可能由神经元的“三元组”组成（具有干燥-冷-和潮湿-空气响应细胞），但IR 40 a表达仅包括冷-和干燥-空气细胞。在这里，我们报告的识别的湿度响应神经元，完成了果蝇触角中的湿觉三联体。这种细胞类型表达Ir 68 a基因，并且Ir 68 a突变扰乱湿度偏好。接下来，我们跟踪Ir 68 a神经元到大脑的投射，并显示它们在触角后叶（PAL）中形成了一个独特的肾小球。在PAL中，一个简单的感觉地图代表了外部环境的相关特征，包括邻近的“热”、“冷”、“干”和“湿”肾小球--一种允许中央中继神经元进行独特和组合采样的组织。事实上，苍蝇对干热的回避比湿热更强烈，这种调节通过沉默干燥空气受体而被消除。一致的是，至少有一个投射神经元类型接受直接突触输入的温度和干燥的空气肾小球。我们的研究结果进一步了解了果蝇触角中的湿度感知，揭示了大脑中温度和湿度的早期感觉整合的神经元基质，并说明了如何在行为学上相关的感觉线索组合可以一起处理以产生适应性行为反应的逻辑。以前的工作确定了干燥空气受体，他们现在表明，潮湿和干燥的细胞与大脑中的热敏神经元汇合，为环境参数创建了一个感觉地图。他们还描述了二阶神经元，采样多种模式的早期整合
The Drosophila antenna contains receptor neurons for mechanical, olfactory, thermal and humidity stimuli. Neurons expressing the ionotropic receptor IR40a have been implicated in the selection of an appropriate humidity range, but while previous work indicates that insect hygroreceptors may be made up by a ‘triad’ of neurons (with a dry- a cold- and a humid-air responding cell), IR40a expression included only cold- and dry-air cells. Here, we report the identification of the humid-responding neuron that completes the hygrosensory triad in the Drosophila antenna. This cell type expresses the Ir68a gene, and Ir68a mutation perturbs humidity preference. Next, we follow the projections of Ir68a neurons to the brain, and show that they form a distinct glomerulus in the posterior antennal lobe (PAL). In the PAL, a simple sensory map represents related features of the external environment with adjacent ‘hot’, ‘cold’, ‘dry’, and ‘humid’ glomeruli -an organization that allows for both unique and combinatorial sampling by central relay neurons. Indeed, flies avoided dry heat more robustly than humid heat, and this modulation was abolished by silencing dry-air receptors. Consistently, at least one projection neuron type received direct synaptic input from both temperature and dry air glomeruli. Our results further our understanding of humidity sensing in the Drosophila antenna, uncover a neuronal substrate for early sensory integration of temperature and humidity in the brain, and illustrate the logic of how ethologically relevant combinations of sensory cues can be processed together to produce adaptive behavioral responses Frank et al. describe humid-air receptors in the fly antenna. Previous work identified dry-air receptors, and they now show that humid and dry cells converge with thermosensory neurons in the brain, creating a sensory map for environmental parameters. They also describe second-order neurons that sample multiple modalities for early integration
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