Confeito-like assembly of organosilicate-caged fluorophores: ultrabright suprananoparticles for fluorescence imaging.
Confeito-like assembly of organosilicate-caged fluorophores: ultrabright suprananoparticles for fluorescence imaging.
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DOI：
10.1088/0957-4484/23/17/175601
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发表时间：
2012-05-04
期刊：
影响因子：
3.5
通讯作者：
Gangopadhyay S
中科院分区：
文献类型：
--
作者：
Bok S;Korampally V;Polo-Parada L;Mamidi V;Baker GA;Gangopadhyay K;Folk WR;Dasgupta PK;Gangopadhyay S
我们报告了由数百个3.3±0.9 nm单元组装而成的超明亮、光稳定的亚25 nm纳米颗粒团聚体(超纳米颗粒)，每个单元平均包含一个包裹在薄有机硅酸盐笼子中的罗丹明6G(Rh6G)染料分子。这些单独的Rh6G掺杂纳米颗粒(DOSNP)单元由一个包含染料的疏水核心和一个超薄的共形硅酸盐外壳组成，该外壳经过二氧化碳等离子体修饰，可提供有益的“笼子效应”和表面亲水性。在最终的22±5 nm凝聚体中，染料在单个DOSNP单元内的隔离避免了二聚化和相关的自发分子相互作用，否则会导致紧密共存的荧光团的自猝灭。由此得到的超纳米颗粒的亮度比相同体积的游离Rh6G分子高200倍以上。没有可观察到的染料渗出，标签对光漂白的抵抗力是溶液中游离Rh6G的20倍。我们展示了DOSNPs在生物成像应用中作为标记的吸引人的特征。
We report ultrabright, photostable, sub-25 nm nanoparticle agglomerates (suprananoparticles) assembled from a few hundred 3.3 ± 0.9 nm units, each hosting on average a single rhodamine 6G (Rh6G) dye molecule encased in a thin organosilicate cage. These individual Rh6G-doped nanoparticle (DOSNP) units consist of a hydrophobic core containing the dye and an ultrathin, conformal silicate shell modified by CO2 plasma to confer a beneficial “cage effect” as well as surface hydrophilicity. The isolation of the dye within individual DOSNP units in the final 22 ± 5 nm agglomerate avoids dimerization and related spontaneous molecular interactions that otherwise lead to self-quenching in closely co-localized fluorophores. The resulting suprananoparticles are over 200 times brighter than the free Rh6G molecules in the same volume. There is no observable dye leaching, and the labels are 20-fold more resistant to photobleaching than free Rh6G in solution. We demonstrate the attractive features of DOSNPs as labels in bioimaging applications.
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