Intervention of triethylamine on Dunaliella tertiolecta reveals metabolic insights into triacylglycerol accumulation
Intervention of triethylamine on Dunaliella tertiolecta reveals metabolic insights into triacylglycerol accumulation
复制标题
三乙胺对杜氏盐藻的干预揭示了三酰甘油积累的代谢见解
DOI：
10.1016/j.algal.2020.101876
复制
发表时间：
2020-05
影响因子：
5.1
通讯作者：
Jiang Jian-Guo
中科院分区：
文献类型：
--
作者：
Chen Hao-Hong;Xue Lu-Lu;Liang Ming-Hua;Jiang Jian-Guo
杜氏藻是一种高产油的微藻。在这里，我们报告了三乙胺干预的新转录组组装和定量基因表达分析，重点关注参与三酰甘油（TAG）生物燃料前体生产的途径之间的复杂相互作用。在100 ppm三乙胺下生长后，我们定量了主要生物分子的细胞含量，包括总脂质、单细胞油含量和脂质组分。转录组重新测序，并通过将读数映射到转录组来定量主要功能类别、相关途径和重要基因的表达。在Illumina HiSeq测序平台上产生了近65，925个高质量读数。除了观察到的脂肪酸合成途径的过度表达，TAG生产过程中三乙胺不是通过肯尼迪途径，但旁路途径高表达的磷酸：二酰基甘油酰基转移酶。糖酵解途径也被下调，不仅在细胞质中，而且在叶绿体中。磷酸丙糖转运蛋白表达下调，DNA与One Finger蛋白基因的结合上调。定量转录组研究揭示了三乙胺胁迫如何导致杜氏藻产生过量TAG的广泛概述，并提供了各种基因工程目标和策略，以改善含油微藻中的生物燃料前体。
Dunaliellatertiolectais a highly oil-producing microalgae. Here, we report itsde novotranscriptome assembly and quantitative gene expression analysis of the intervention of triethylamine, with a focus on the complex interactions of pathways involved in the production of triacylglycerol (TAG) biofuel precursors. After growing under 100 ppm triethylamine, we quantified the cellular content of the major biomolecules, including total lipids, single cell oil content, and lipid components. The transcriptome was assembledde novoand the main functional classes, related pathways, and expression of important genes were quantified by the mapping of reads to the transcriptome. Almost 65,925 high-quality readings were produced on the Illumina HiSeq sequencing platform. In addition to the observed overexpression of the fatty acid synthesis pathway, TAG production during triethylamine was not through the Kennedy pathway, but the bypass pathway by high expression of phosphate: diacylglycerol acyltransferase. The glycolytic pathway has also been down-regulated, not only in the cytoplasm but also in the chloroplast. Triosephosphate translocator was downregulated, and the DNA binding with One Finger protein gene was upregulated. The quantitative transcriptome study reveals a broad overview of how triethylamine stress leads to excessive TAG production inDunaliella, and provides various genetic engineering goals and strategies to improve biofuel precursors in oleaginous microalgae.
登录
查看更多内容
影响因子：
5.1
作者：
T. Zalogin;U. Pick
通讯作者：
T. Zalogin;U. Pick
影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.7
作者：
Wan L;Han J;Sang M;Li A;Wu H;Yin S;Zhang C
通讯作者：
Zhang C
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y