Osteopontin reduces biofilm formation in a multi-species model of dental biofilm.
Osteopontin reduces biofilm formation in a multi-species model of dental biofilm.
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DOI：
10.1371/journal.pone.0041534
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Meyer RL
中科院分区：
文献类型：
--
作者：
Schlafer S;Raarup MK;Wejse PL;Nyvad B;Städler BM;Sutherland DS;Birkedal H;Meyer RL
对抗牙齿生物膜的形成是预防龋病的最有效手段，龋病是人类最普遍的疾病之一。在机械清洁牙齿的化学添加剂中，针对细菌生物膜形成机制的非杀菌添加剂近年来受到越来越多的关注。牛奶蛋白，如乳铁蛋白，已被证明干扰唾液涂层表面的细菌定植。我们在这里研究了牛乳骨桥蛋白(OPN)，一种高度磷酸化的乳清糖蛋白，在体外多物种牙科生物膜模型中的作用。虽然相当多的研究集中在OPN与哺乳动物细胞的相互作用上，但还没有数据研究OPN对细菌生物膜的影响。将口腔链球菌、奈氏放线菌、中型链球菌、唐氏链球菌和血链球菌组成的生物膜培养在可进行原位显微镜分析的流动细胞系统中。结晶紫染色显示，与没有OPN的生物膜或在酪蛋白糖巨肽(另一种磷酸化的牛奶蛋白)存在的生物膜相比，OPN存在时生物膜的形成明显减少。共聚焦显微镜显示，OPN结合在细菌细胞表面，降低了生物膜的机械稳定性，而不影响细胞活力。通过荧光原位杂交测定，在OPN存在下，生物膜的细菌组成发生了很大的变化。特别是，模型中最好的生物膜形成者米氏葡萄球菌的定殖率显著减少。OPN大大减少了在定义良好的产酸牙科生物膜的实验室模型中形成的生物膜的数量。如果在活体中也能观察到类似的效果，OPN可能会作为机械清洁牙齿程序的一个有价值的辅助手段。
Combating dental biofilm formation is the most effective means for the prevention of caries, one of the most widespread human diseases. Among the chemical supplements to mechanical tooth cleaning procedures, non-bactericidal adjuncts that target the mechanisms of bacterial biofilm formation have gained increasing interest in recent years. Milk proteins, such as lactoferrin, have been shown to interfere with bacterial colonization of saliva-coated surfaces. We here study the effect of bovine milk osteopontin (OPN), a highly phosphorylated whey glycoprotein, on a multispecies in vitro model of dental biofilm. While considerable research effort focuses on the interaction of OPN with mammalian cells, there are no data investigating the influence of OPN on bacterial biofilms. Biofilms consisting of Streptococcus oralis, Actinomyces naeslundii, Streptococcus mitis, Streptococcus downei and Streptococcus sanguinis were grown in a flow cell system that permitted in situ microscopic analysis. Crystal violet staining showed significantly less biofilm formation in the presence of OPN, as compared to biofilms grown without OPN or biofilms grown in the presence of caseinoglycomacropeptide, another phosphorylated milk protein. Confocal microscopy revealed that OPN bound to the surface of bacterial cells and reduced mechanical stability of the biofilms without affecting cell viability. The bacterial composition of the biofilms, determined by fluorescence in situ hybridization, changed considerably in the presence of OPN. In particular, colonization of S. mitis, the best biofilm former in the model, was reduced dramatically. OPN strongly reduces the amount of biofilm formed in a well-defined laboratory model of acidogenic dental biofilm. If a similar effect can be observed in vivo, OPN might serve as a valuable adjunct to mechanical tooth cleaning procedures.
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影响因子：
3.7
作者：
Schlafer S;Raarup MK;Meyer RL;Sutherland DS;Dige I;Nyengaard JR;Nyvad B
通讯作者：
Nyvad B
影响因子：
3.1
作者：
CISAR, JO;KOLENBRANDER, PE;MCINTIRE, FC
通讯作者：
MCINTIRE, FC
影响因子：
--
作者：
Arslan, S. Y.;Leung, K. P.;Wu, C. D.
通讯作者：
Wu, C. D.
影响因子：
4.2
作者：
Guggenheim, B;Schmid, R;Neeser, JR
通讯作者：
Neeser, JR
影响因子：
4.9
作者：
Wakabayashi, Hiroyuki;Yamauchi, Koji;Yoshie, Hiromasa
通讯作者：
Yoshie, Hiromasa