Biosynthetic labeling with 3-O-propargylcaffeyl alcohol reveals in vivo cell-specific patterned lignification in loquat fruits during development and postharvest storage.
Biosynthetic labeling with 3-O-propargylcaffeyl alcohol reveals in vivo cell-specific patterned lignification in loquat fruits during development and postharvest storage.
复制标题
DOI：
10.1038/s41438-021-00497-z
复制
发表时间：
2021-03-10
影响因子：
8.7
通讯作者：
Chen K
中科院分区：
文献类型：
--
作者：
Zhu N;Zhao C;Wei Y;Sun C;Wu D;Chen K
木质化是一种主要的细胞壁修饰，其通常导致在植物发育期间或响应于环境胁迫而形成复杂的亚细胞模式。精确定位木质素的时空沉积对于揭示单个细胞的木质化调控机制具有重要意义。在枇杷果实中，木质化通常增加果肉木质素含量和硬度，降低其食用性和加工品质。然而，枇杷果肉内的木质化的时空活动区的精确定位仍然知之甚少，并且很少有人知道图案化木质化对细胞壁结构动态和随后的果实品质劣化的贡献。在这里，我们进行了一个新兴的生物正交化学成像技术跟踪在体内模式的枇杷果肉细胞在发育和贮藏过程中的木质化动态。在果实发育过程中，木质素细胞（LC）和维管束（VB）是木质化活跃的区域，环状LC在内壁层和外周侧沉积木质素。初步观察到了液晶产生的多米诺效应。在成熟果实中，贮藏期间果肉中新形成的木质素特异性地沉积在VBs周围薄壁细胞的角部和中层，导致网状结构的发育。在此基础上，提出了枇杷果实不同果肉细胞木质化的时空模式。这些研究结果提供了枇杷木质化动态和时空数据，可以提高我们对植物木质化过程的理解。
Lignification is a major cell wall modification that often results in the formation of sophisticated subcellular patterns during plant development or in response to environmental stresses. Precise localization of the spatiotemporal deposition of lignin is of great importance for revealing the lignification regulatory mechanism of individual cells. In loquat fruits, lignification typically increases the flesh lignin content and firmness, reducing their edibility and processing quality. However, the precise localization of the spatiotemporal active zones of lignification inside loquat fruit flesh remains poorly understood, and little is known about the contribution of patterned lignification to cell wall structure dynamics and the subsequent fruit-quality deterioration. Here, we performed an emerging bioorthogonal chemistry imaging technique to trace the in vivo patterned lignification dynamics in cells of loquat fruit flesh during development and storage. In developing fruits, lignified cells (LCs) and vascular bundles (VBs) were the zones of active lignification, and ring-like LCs deposited lignin at both the inner wall layer and the outer periphery sides. The domino effect of the generation of LCs was preliminarily visualized. In mature fruits, the newly formed lignin in the flesh of fruits during storage was specifically deposited in the corners and middle lamellae of parenchyma cells surrounding the VBs, resulting in the development of a reticular structure. Based on the findings, distinct spatiotemporal patterned lignification modes for different flesh cells in loquat fruits were proposed. These findings provide loquat lignification dynamics together with spatiotemporal data that can improve our understanding of the lignification process in planta.
登录
查看更多内容
影响因子：
1.2
作者：
Mitra, Prajakta Pradhan;Loque, Dominique
通讯作者：
Loque, Dominique
影响因子：
1.9
作者：
Kim, Jong Sik;Daniel, Geoffrey
通讯作者：
Daniel, Geoffrey
影响因子：
7
作者：
Kamdee, Chanattika;Imsabai, Wachiraya;Ketsa, Saichol
通讯作者：
Ketsa, Saichol
EjNAC3 通过与非典型 CAD 样基因的物理相互作用转录调节枇杷果实的冷藏诱导木质化
影响因子：
6.9
作者：
Ge H;Zhang J;Zhang YJ;Li X;Yin XR;Grierson D;Chen KS
通讯作者：
Chen KS
影响因子：
7.4
作者：
Araujo, Pedro;Ferreira, Monica Siqueira;Mazzafera, Paulo
通讯作者：
Mazzafera, Paulo