Enhancing structural plasticity of PC12 neurons during differentiation and neurite regeneration with a catalytically inactive mutant version of the zRICH protein.
Enhancing structural plasticity of PC12 neurons during differentiation and neurite regeneration with a catalytically inactive mutant version of the zRICH protein.
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DOI：
10.1186/s12868-023-00808-1
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发表时间：
2023-08-23
期刊：
影响因子：
2.4
通讯作者：
Ballestero, Rafael P.
中科院分区：
文献类型：
--
作者：
Bandla, Ashoka C.;Sheth, Aditya S.;Zarate, Sara M.;Uskamalla, Suraj;Hager, Elizabeth C.;Villarreal, Victor A.;Gonzalez-Garcia, Maribel;Ballestero, Rafael P.
关键词：
Studies of the molecular mechanisms of nerve regeneration have led to the discovery of several proteins that are induced during successful nerve regeneration. RICH proteins were identified as proteins induced during the regeneration of the optic nerve of teleost fish. These proteins are 2’,3’-cyclic nucleotide, 3’-phosphodiesterases that can bind to cellular membranes through a carboxy-terminal membrane localization domain. They interact with the tubulin cytoskeleton and are able to enhance neuronal structural plasticity by promoting the formation of neurite branches. PC12 stable transfectant cells expressing a fusion protein combining a red fluorescent protein with a catalytically inactive mutant version of zebrafish RICH protein were generated. These cells were used as a model to analyze effects of the protein on neuritogenesis. Differentiation experiments showed a 2.9 fold increase in formation of secondary neurites and a 2.4 fold increase in branching points. A 2.2 fold increase in formation of secondary neurites was observed in neurite regeneration assays. The use of a fluorescent fusion protein facilitated detection of expression levels. Two computer-assisted morphometric analysis methods indicated that the catalytically inactive RICH protein induced the formation of branching points and secondary neurites both during differentiation and neurite regeneration. A procedure based on analysis of random field images provided comparable results to classic neurite tracing methods. The online version contains supplementary material available at 10.1186/s12868-023-00808-1.
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影响因子：
8.2
作者：
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通讯作者：
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影响因子：
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作者：
Challa, Madhavi;Chapa, Gloria R.;Ballestero, Rafael P.
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DOI：
10.1038/s41583-020-0314-2
发表时间：
2020-07
期刊：
Nature reviews. Neuroscience
影响因子：
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通讯作者：
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影响因子：
4.6
作者：
Baker EW;Platt SR;Lau VW;Grace HE;Holmes SP;Wang L;Duberstein KJ;Howerth EW;Kinder HA;Stice SL;Hess DC;Mao H;West FD
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West FD