Ribose-phosphate pyrophosphokinase 1 (PRPS1) associated with deltamethrin resistance in Culex pipiens pallens.
Ribose-phosphate pyrophosphokinase 1 (PRPS1) associated with deltamethrin resistance in Culex pipiens pallens.
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DOI：
10.1007/s00436-012-3205-2
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发表时间：
2013-02
影响因子：
2
通讯作者：
Zhu C
中科院分区：
文献类型：
--
作者：
Hong S;Zhou D;Chen C;Wang W;Lv Y;Ye Y;Zou P;Yv J;Chang X;Yv X;Shi L;Ma L;Sun Y;Zhang D;Shen B;Zhu C
应用基因芯片和2D-Gel技术鉴定和分离了溴氰菊酯敏感(DS)和抗性(DR)淡色库蚊和白纹伊蚊C6/36细胞系的差异表达基因--核糖-磷酸焦磷酸激酶1(PRPS1)。从淡色库蚊中克隆的PRPS1开放阅读框全长1,011个核苷酸，编码336个氨基酸，与致倦库蚊高度同源。用实时荧光定量聚合酶链式反应检测DS株和DR株PRPS1的转录表达水平。PRPS1在DR实验室株和自然人群JXZ-DR、JXZ-LDR中表达水平较高。PRPS1在淡色库蚊各发育阶段均有表达，在3龄、4龄幼虫、雌虫和雄虫中，DR3品系的表达水平高于DS品系。此外，为了进一步研究PRPS1在溴氰菊酯抗性中的作用，我们在白纹伊蚊C6/36细胞中瞬时表达了PRPS1，并用免疫印迹法检测了PRPS1的表达。与对照细胞相比，PRPS1转基因细胞对溴氰菊酯的抗性增强。这些结果表明，PRPS1表达水平的升高可能在溴氰菊酯抗性的调节中起作用。
Ribose-phosphate pyrophosphokinase 1 (PRPS1) was identified and isolated as a differentially expressed gene between deltamethrin-susceptible (DS) and deltamethrin-resistant (DR) Culex pipiens pallens and Aedes albopictus C6/36 cell line through microarray and 2D-Gel. An open reading frame of PRPS1 cloned from C. pipiens pallens has 1,011 bp and encodes for a 336 amino acids protein which shares high homology with Culex quinquefasciatus. Real-time polymerase chain reaction was used to determine the transcript expression level of PRPS1 in DS and DR strains. The expression levels of PRPS1 were higher in DR laboratory strains and natural population JXZ-DR, JXZ-LDR. PRPS1 was also detected and expressed at all developmental stages of C. pipiens pallens and increased expression level in DR3 strain than DS strain in the third and fourth instar larvae, female and male stages. In addition, to further investigate the role of PRPS1 in deltamethrin resistance, PRPS1 was transiently expressed in A. albopictus C6/36 cells and detected by western blotting. Cells transfected with PRPS1 had an increased resistance to deltamethrin compared with control cells. These results suggested that the increased expression level of PRPS1 may play roles in the regulation of deltamethrin resistance.
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影响因子：
3.7
作者：
Chen L;Zhong D;Zhang D;Shi L;Zhou G;Gong M;Zhou H;Sun Y;Ma L;He J;Hong S;Zhou D;Xiong C;Chen C;Zou P;Zhu C;Yan G
通讯作者：
Yan G
影响因子：
3.8
作者：
Burton, Mark J.;Mellor, Ian R.;Williamson, Martin S.
通讯作者：
Williamson, Martin S.
影响因子：
3.1
作者：
Pasteur, N;Raymond, M
通讯作者：
Raymond, M
DOI：
10.2987/8756-971x(2007)23
发表时间：
2007-01-01
影响因子：
1
作者：
Platzer, Edward G. -
通讯作者：
Platzer, Edward G. -
影响因子：
2.6
作者：
Vontas, J;Blass, C;Ranson, H
通讯作者：
Ranson, H