The effect of carbohydrate restriction on prostate cancer tumor growth in a castrate mouse xenograft model.
The effect of carbohydrate restriction on prostate cancer tumor growth in a castrate mouse xenograft model.
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DOI：
10.1002/pros.22586
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发表时间：
2013-04
期刊：
影响因子：
2.8
通讯作者：
Freedland, Stephen J.
中科院分区：
文献类型：
--
作者：
Caso, Jorge;Masko, Elizabeth M.;Thomas, Jean A., II;Poulton, Susan H.;Dewhirst, Mark;Pizzo, Salvatore V.;Freedland, Stephen J.
在前列腺癌（PCa）异种移植研究中，与西方饮食（WD）相比，无碳水化合物和低碳水化合物饮食可延迟肿瘤生长。方法：共160只雄性SCID小鼠注射1 × 105LAPC-4人前列腺癌细胞。其中，将150只小鼠去势并随机分配至自由采食WD或通过配对喂养方案喂食无碳水化合物生酮饮食（NCKD）、10%碳水化合物饮食或20%碳水化合物饮食。其余10只小鼠未去势，并随意饲喂WD。一旦体积达到1，000 mm3，处死小鼠，并使用对数秩检验检测存活率。对中位存活8只小鼠/组的血清进行胰岛素、IGF-1和IGFBP-3测定。各组间体重大致相等。10只非去势小鼠出现肿瘤生长加速。在去势小鼠中，WD的肿瘤生长最快，20%碳水化合物饮食最慢（P = 0.046）。不同碳水化合物限制组之间的生存率无显著差异（P = 0.51）。合并时，碳水化合物限制饮食与WD相比，生存率改善的趋势不显著（P = 0.11）。在随机分组前或处死时，所有组的血清胰岛素、IGF-1和IGFBP-3水平无显著差异。虽然碳水化合物限制的益处略低于先前在非去势小鼠中的研究，但这些数据仍然表明人类可实现的饮食可能在PCa管理中发挥作用。前列腺73：449 - 454，2013年。© 2012 Wiley Periodicals，Inc.
BACKGROUNDNo‐ and low‐carbohydrate diets delay tumor growth compared to western diet (WD) in prostate cancer (PCa) xenograft studies. The effect of these diets in concert with androgen deprivation is unknown.METHODSA total of 160 male SCID mice were injected with 1× 105LAPC‐4 human PCa cells. Of these, 150 mice were castrated and randomized to an ad libitum WD or fed via a paired‐feeding protocol with a no‐carbohydrate ketogenic diet (NCKD), 10% carbohydrate diet, or 20% carbohydrate diet. The remaining 10 mice were not castrated and were fed an ad libitum WD. The mice were sacrificed once volumes reached 1,000 mm3and survival tested using the log‐rank test. Serum from the median surviving 8 mice/group was assayed for insulin, IGF‐1, and IGFBP‐3.RESULTSBody weights were roughly equal among groups. The 10 non‐castrated mice experienced accelerated tumor growth. Among castrated mice, WD had the most rapid tumor growth; 20% carbohydrate diet the slowest (P= 0.046). Survival was not significantly different among the various carbohydrate restricted groups (P= 0.51). When pooled, there was a non‐significant trend (P= 0.11) in improved survival among the carbohydrate restricted diets versus WD. No significant difference in serum insulin, IGF‐1, and IGFBP‐3 levels was noted among all groups at pre‐randomization or at sacrifice.CONCLUSIONSA 20% carbohydrate diet slowed tumor growth versus a WD. Though the benefit of carbohydrate restriction was somewhat less than in prior studies in non‐castrate mice, these data still suggest diets achievable in humans may play a role in PCa management. Prostate 73: 449–454, 2013. © 2012 Wiley Periodicals, Inc.
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影响因子：
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通讯作者：
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