Effects of crosslinking on the mechanical properties, drug release and cytocompatibility of protein polymers.
Effects of crosslinking on the mechanical properties, drug release and cytocompatibility of protein polymers.
复制标题
DOI：
10.1016/j.actbio.2013.08.029
复制
发表时间：
2014-01
影响因子：
9.7
通讯作者：
Chaikof, Elliot L.
中科院分区：
文献类型：
--
作者：
Martinez, Adam W.;Caves, Jeffrey M.;Ravi, Swathi;Li, Wehnsheng;Chaikof, Elliot L.
重组类弹性蛋白聚合物作为各种植入式医疗器械的组件材料正受到越来越多的研究。这主要是因为它们具有良好的生物学特性，并具有调整其物理和机械特性的能力。在这份报告中，我们探索了通过选择不同的交联方案和加工策略来调节蛋白质膜的水分含量、机械性能和药物释放曲线的潜力。我们发现，交联方案和加工策略的选择对蛋白质聚合物薄膜的各个方面都有很大的影响。值得注意的是，利用受限、固定体积以及气相交联策略降低了蛋白质聚合物的平衡水含量。具体地说，与未交联的蛋白质凝胶相比，京尼平(15.5%)、戊二醛(GTA)(24.5%)、GTA蒸汽交联(31.6%)、二硫化物(SS)(18.2%)和SS蒸汽交联(25.5%)的水分含量降低(p<；0.05)。不同的交联策略调节了蛋白质聚合物的硬度、破坏时的应变和极限拉伸强度(UTS)。在所有情况下，气相交联产生的薄膜最坚硬，具有最高的UTS。此外，受限、固定体积和气相方法都影响药物传递速率；与溶液相交联相比，初始药物突发和释放速率降低。定制的交联策略为调节蛋白质聚合物的物理、机械和药物输送特性提供了一个重要的选择。
Recombinant elastin-like protein polymers are increasingly being investigated as component materials of a variety of implantable medical devices. This is chiefly a result of their favorable biological properties and the ability to tailor their physical and mechanical properties. In this report, we explore the potential of modulating the water content, mechanical properties, and drug release profiles of protein films through the selection of different crosslinking schemes and processing strategies. We find that the selection of crosslinking scheme and processing strategy has a significant influence on all aspects of protein polymer films. Significantly, utilization of a confined, fixed volume, as well as vapor phase crosslinking strategies decreased protein polymer equilibrium water content. Specifically, as compared to uncrosslinked protein gels, water content was reduced for genipin (15.5%), glutaraldehyde (GTA) (24.5%), GTA vapor crosslinking (31.6%), disulfide (SS) (18.2%), and SS vapor crosslinking (25.5%) (p <0.05). Distinct crosslinking strategies modulated protein polymer stiffness, strain at failure, and ultimate tensile strength (UTS). In all cases, vapor phase crosslinking produced the stiffest films with the highest UTS. Moreover, both confined, fixed volume and vapor phase approaches influenced drug delivery rates; with decreased initial drug burst and release rates as compared to solution phase crosslinking. Tailored crosslinking strategies provide an important option for modulating the physical, mechanical, and drug delivery properties of protein polymers.
登录
查看更多内容
DOI：
10.1002/jbm.820240905
发表时间：
1990-09-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
HUANGLEE, LLH;CHEUNG, DT;NIMNI, ME
通讯作者：
NIMNI, ME
影响因子：
14
作者：
Caves, Jeffrey M.;Kumar, Vivek A.;Martinez, Adam W.;Kim, Jeong;Ripberger, Carrie M.;Haller, Carolyn A.;Chaikof, Elliot L.
通讯作者：
Chaikof, Elliot L.
影响因子：
2.9
作者：
Bellingham, CM;Lillie, MA;Keeley, FW
通讯作者：
Keeley, FW
影响因子：
14
作者：
Nicolas, FL;Gagnieu, CH
通讯作者：
Gagnieu, CH
DOI：
10.1002/jbm.10145
发表时间：
2002-07-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Gough, JE;Scotchford, CA;Downes, S
通讯作者：
Downes, S