Hydrogen Sulfide Promotes Wheat Grain Germination Under Cadmium Stress
Hydrogen Sulfide Promotes Wheat Grain Germination Under Cadmium Stress
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镉胁迫下硫化氢促进小麦籽粒发芽
DOI：
10.1007/s40011-015-0554-5
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Zhang, Hua
中科院分区：
文献类型：
--
作者：
Yan, Hong;Li, Wan-Jie;Jiao, Hao;Zhang, Hua
硫化氢（H2S）是一种多功能的信号分子，参与植物的生长发育和对非生物胁迫的应答。本文研究了H2S供体硫氢化钠（NaHS）对小麦发芽的影响。研究了镉胁迫下水稻籽粒的生长状况。随着Cd浓度的增加，小麦籽粒的发芽率逐渐降低。NaHS预处理缓解了镉诱导的萌发抑制，并呈剂量依赖性，最佳浓度为0.9 mM。进一步的研究表明，NaHS预处理可以提高小麦种子萌发过程中淀粉酶和酯酶的活性，减轻镉对小麦胚根根尖质膜完整性的影响，维持较低的超氧自由基、过氧化氢和丙二醛水平。此外，NaHS显著促进了镉胁迫下籽粒中游离氨基酸的积累。进一步研究表明，NaHS预处理促进了过氧化物酶、过氧化氢酶和抗坏血酸过氧化物酶的活性，抑制了脂氧合酶的活性。此外，在NaHS预处理的小麦籽粒中观察到较高水平的内源H2S。这些数据表明，H2S改善镉胁迫发芽小麦籽粒通过调节抗氧化系统。
Hydrogen sulfide (H2S) has proved to be a multifunctional signaling molecule involved in plant development and response to abiotic stresses. In the present study, the effects of H2S donor, sodium hydrosulfide (NaHS) on the germination of wheat (Triticum aestivumL.) grains under cadmium stress was investigated. With the increase in Cd concentration, the germination percentage of wheat grains decreased steadily. NaHS pretreatment alleviated cadmium-induced germination inhibition in a dose-dependent manner, with the optimal concentration of 0.9 mM. Further studies showed that NaHS pretreatment could promote the activities of amylase and esterase, reducing cadmium-induced disturbance of plasma membrane integrity in the radicle tips, and sustain lower levels of superoxide radicals, hydrogen peroxide and malondialdehyde in germinating wheat grains. Besides, NaHS significantly promoted the accumulation of free amino acids in grains exposed to cadmium. Further investigation showed that NaHS pretreatment stimulated the activities of peroxidase, catalase and acorbate peroxidase and inhibited that of lipoxygenase. In addition, a higher level of endogenous H2S in wheat grains was observed in NaHS-pretreated wheat grains. These data indicate that H2S ameliorated cadmium-stress in germinating wheat grains by modulating the antioxidant system.
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DOI：
10.1016/j.plaphy.2013.01.003
发表时间：
2013-04
期刊：
Plant physiology and biochemistry : PPB
影响因子：
--
作者：
Jian Sun;Rui-gang Wang;Xuan Zhang;Yicheng Yu;R. Zhao;Zongyun Li;Shaoliang Chen
通讯作者：
Jian Sun;Rui-gang Wang;Xuan Zhang;Yicheng Yu;R. Zhao;Zongyun Li;Shaoliang Chen
影响因子：
11.4
作者：
Hua Zhang;Zhu-Qin Tan;Lan-Ying Hu;Song-hua Wang;Jian-ping Luo;Russell L. Jones
通讯作者：
Hua Zhang;Zhu-Qin Tan;Lan-Ying Hu;Song-hua Wang;Jian-ping Luo;Russell L. Jones
影响因子：
7.4
作者：
Yoko Yamamoto;Yukiko Kobayashi;H. Matsumoto
通讯作者：
Yoko Yamamoto;Yukiko Kobayashi;H. Matsumoto
影响因子：
2.7
作者：
García-Limones, C;Hervás, A;Tena, M
通讯作者：
Tena, M
影响因子：
7.3
作者：
Romero-Puertas, MC;Palma, JM;Sandalio, LM
通讯作者：
Sandalio, LM