Gene repertoire evolution of Streptococcus pyogenes inferred from phylogenomic analysis with Streptococcus canis and Streptococcus dysgalactiae.
Gene repertoire evolution of Streptococcus pyogenes inferred from phylogenomic analysis with Streptococcus canis and Streptococcus dysgalactiae.
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从系统基因分析的基因库曲霉的基因演变，癌链球菌和性链球菌链球菌的进化。
DOI：
10.1371/journal.pone.0037607
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Stanhope MJ
中科院分区：
文献类型：
--
作者：
Lefébure T;Richards VP;Lang P;Pavinski-Bitar P;Stanhope MJ
化脓性链球菌是一种重要的人类病原体，属于化脓性链球菌类，专门适应人类宿主。我们的目标是采用比较进化方法来更好地了解与化脓性链球菌人类适应相关的基因组事件。作为确定这些事件的一部分，我们对潜在姐妹物种之一——农业病原体犬链球菌的基因组进行了测序，并将其与另外两个密切相关的物种——停乳链球菌和马链球菌——进行比较基因组协调分析，以确定化脓性链球菌进化过程中获得和丢失的基因。涉及 15 种链球菌的全基因组系统发育分析为马链球菌、化脓性链球菌、停乳链球菌和犬链球菌的分支提供了令人信服的支持，并表明最可能的化脓性链球菌姐妹种是停乳链球菌。协调分析确定了在化脓性链球菌谱系中获得的 113 个基因。这些获得的基因中几乎一半 (46%) 与噬菌体相关，其中 14 个基因与实验验证的细菌毒力因子显着匹配。在化脓性链球菌起源之后，超过一半的噬菌体相关基因参与了90个不同的LGT事件，大多数涉及化脓性链球菌的不同菌株，但其中很大一部分涉及马特异性病原体马链球菌亚种。马，方向性几乎完全（86％）在化脓性链球菌到马链球菌方向。无乳链球菌似乎在化脓性链球菌的进化中发挥了重要作用，其中很大一部分 LGT 源自该物种。总体而言，分析表明，化脓性链球菌对人类宿主的适应部分是通过（i）新毒力因子（例如 speB 和 sal 位点）的整合和（ii）新调控网络（例如 rgg 和某种程度上 speB）的构建来实现的。
Streptococcus pyogenes, is an important human pathogen classified within the pyogenic group of streptococci, exclusively adapted to the human host. Our goal was to employ a comparative evolutionary approach to better understand the genomic events concomitant with S. pyogenes human adaptation. As part of ascertaining these events, we sequenced the genome of one of the potential sister species, the agricultural pathogen S. canis, and combined it in a comparative genomics reconciliation analysis with two other closely related species, Streptococcus dysgalactiae and Streptococcus equi, to determine the genes that were gained and lost during S. pyogenes evolution. Genome wide phylogenetic analyses involving 15 Streptococcus species provided convincing support for a clade of S. equi, S. pyogenes, S. dysgalactiae, and S. canis and suggested that the most likely S. pyogenes sister species was S. dysgalactiae. The reconciliation analysis identified 113 genes that were gained on the lineage leading to S. pyogenes. Almost half (46%) of these gained genes were phage associated and 14 showed significant matches to experimentally verified bacteria virulence factors. Subsequent to the origin of S. pyogenes, over half of the phage associated genes were involved in 90 different LGT events, mostly involving different strains of S. pyogenes, but with a high proportion involving the horse specific pathogen S. equi subsp. equi, with the directionality almost exclusively (86%) in the S. pyogenes to S. equi direction. Streptococcus agalactiae appears to have played an important role in the evolution of S. pyogenes with a high proportion of LGTs originating from this species. Overall the analysis suggests that S. pyogenes adaptation to the human host was achieved in part by (i) the integration of new virulence factors (e.g. speB, and the sal locus) and (ii) the construction of new regulation networks (e.g. rgg, and to some extent speB).
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影响因子：
3.7
作者：
Beres SB;Musser JM
通讯作者：
Musser JM
影响因子：
11.8
作者：
Brandt, Claudia M.;Spellerberg, Barbara
通讯作者：
Spellerberg, Barbara
影响因子：
48
作者：
Bejerano, G;Siepel, AC;Haussler, D
通讯作者：
Haussler, D
影响因子：
28.2
作者：
Galperine, Tatiana;Cazorla, Cecile;Neau, Didier
通讯作者：
Neau, Didier
影响因子：
3
作者：
COLMAN, G;TANNA, A;GAWORZEWSKA, ET
通讯作者：
GAWORZEWSKA, ET