CD11c+ M1-like macrophages (MΦs) but not CD206+ M2-like MΦ are involved in folliculogenesis in mice ovary.
CD11c+ M1-like macrophages (MΦs) but not CD206+ M2-like MΦ are involved in folliculogenesis in mice ovary.
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DOI：
10.1038/s41598-018-25837-3
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发表时间：
2018-05-25
影响因子：
4.6
通讯作者：
Saito S
中科院分区：
文献类型：
--
作者：
Ono Y;Nagai M;Yoshino O;Koga K;Nawaz A;Hatta H;Nishizono H;Izumi G;Nakashima A;Imura J;Tobe K;Fujii T;Osuga Y;Saito S
巨噬细胞（MΦ）参与卵泡发生和排卵。然而，目前尚不清楚哪种类型的MΦ，M1或M2，在卵巢中发挥更重要的作用。使用CD 206或CD 11 c白喉毒素受体转基因（DTR）小鼠，其能够分别耗尽CD 206 + M2 MΦ和CD 11 c + MΦ或CD 11 c+树突细胞（DC）。卵母细胞用于体外受精和胚胎移植。将来自M2 MΦ耗尽的卵母细胞的体外受精胚胎转移到假怀孕的野生型小鼠。用CD 11 c DTR小鼠研究CD 11 c细胞、M1 MΦ和DC在卵泡发生中的作用。在WT小鼠中，CD 206 + M2-like MΦs的比例在卵泡诱导中未增加，而CD 11 c + M1-like MΦs的比例增加。在CD 206 DTR小鼠中，卵泡发生正常，排卵数、受精率和着床率与WT小鼠相似。在CD 11 c DTR小鼠中，卵巢出血导致卵泡发生受损，血小板衍生生长因子受体β（PDGF-Rβ）（周细胞的标志物）和CD 34（内皮细胞的标志物）的染色减少。CD 11 c+细胞，M1 MΦs或DC，可能参与卵泡发生，而M2 MΦs不参与卵泡发生。
Macrophages (MΦs) are involved in folliculogenesis and ovulation. However, it is unknown which type of MΦ, M1 or M2, plays a more essential role in the ovary. CD206 or CD11c diphtheria toxin receptor transgenic (DTR) mice, which enable depletion of CD206+ M2 MΦs and CD11c+ MΦ or CD11c+ Dendritic cells (DCs), respectively, were used. Oocytes were used for in vitro fertilization and embryo transfer. In vitro fertilized embryos derived from M2 MΦ depleted oocytes were transferred to pseudo pregnant wild type mice. CD11c DTR mice were also used to investigate the role of CD11c cells, M1 MΦ and DCs in folliculogenesis. In WT mice, the proportion of CD206+ M2-like MΦs was not increased in follicular induction, while that of CD11c+ M1-like MΦs was increased. In CD206 DTR mice, folliculogenesis was normal and the ovulation number, fertilization rate, and implantation rate were similar to those in WT mice. In CD11c DTR mice, folliculogenesis was impaired with ovarian hemorrhage and the staining of platelet derived growth factor-receptor β (PDGF-Rβ), a marker of pericytes, and CD34, a marker of endothelial cells, was reduced. CD11c+ cells, M1 MΦs or DCs, may be involved in folliculogenesis, while M2 MΦs are not involved in folliculogenesis.
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影响因子：
6.7
作者：
Geva, E;Jaffe, RB
通讯作者：
Jaffe, RB
DOI：
10.1073/pnas.81.13.4174
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
HUME, DA;HALPIN, D;GORDON, S
通讯作者：
GORDON, S
DOI：
10.1530/jrf.0.0960241
发表时间：
1992-09-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
FUKUMATSU, Y;KATABUCHI, H;OKAMURA, H
通讯作者：
OKAMURA, H
影响因子：
2.1
作者：
Duda, Malgorzata;Knet, Malgorzata;Slomczynska, Maria
通讯作者：
Slomczynska, Maria
影响因子：
2.4
作者：
Inoue, S;Watanabe, H;Tonosaki, A
通讯作者：
Tonosaki, A